A Low Molecular Weight Enterotoxic Hemolysin From Clinical Enterobacter Cloacae.

dc.creatorSimi, S
dc.creatorCarbonell, G V
dc.creatorFalcón, R M
dc.creatorGatti, M S V
dc.creatorJoazeiro, P P
dc.creatorDarini, A L
dc.creatorYano, T
dc.date2003-Jul
dc.date2015-11-27T12:52:30Z
dc.date2015-11-27T12:52:30Z
dc.date.accessioned2018-03-29T00:58:08Z
dc.date.available2018-03-29T00:58:08Z
dc.identifierCanadian Journal Of Microbiology. v. 49, n. 7, p. 479-82, 2003-Jul.
dc.identifier0008-4166
dc.identifier10.1139/w03-060
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/14569289
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/195563
dc.identifier14569289
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1295796
dc.descriptionSeven of 50 Enterobacter cloacae strains from clinical isolates produced small turbid zones of hemolysis in horse and sheep blood agar plates, and the culture supernatants were also positive for hemolytic activity. The hemolysin was partially purified from the culture supernatant of E. cloacae by ultrafiltration (PM-10 membrane) and extraction with acetone. Semipurified hemolysin was stable to heating (100 degrees C, 30 min) and was soluble in organic solvents (acetone, ethanol, and methanol). The toxin showed no loss of biological activity after treatment with trypsin and was stable to acid treatment at pH 2.0 but not at a pH greater than 7.0. In the rat intestinal loop assay, the hemolysin caused hemorrhagic fluid accumulation and severe histological alterations. These findings indicate that this hemolysin may be a putative virulence factor in E. cloacae infections.
dc.description49
dc.description479-82
dc.languageeng
dc.relationCanadian Journal Of Microbiology
dc.relationCan. J. Microbiol.
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectAgar
dc.subjectAnimals
dc.subjectEnterobacter Cloacae
dc.subjectHemolysin Proteins
dc.subjectHemolysis
dc.subjectHorses
dc.subjectHumans
dc.subjectIntestines
dc.subjectMolecular Weight
dc.subjectRats
dc.subjectSheep
dc.subjectVirulence
dc.titleA Low Molecular Weight Enterotoxic Hemolysin From Clinical Enterobacter Cloacae.
dc.typeArtículos de revistas


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