Artículos de revistas
Ciliated Fibroblasts In Mouse Connective Tissues: A Comparison Between Methods For Estimating Their Frequency.
Registro en:
Journal Of Submicroscopic Cytology And Pathology. v. 35, n. 1, p. 83-9, 2003-Jan.
1122-9497
12762656
Autor
Barbosa, S C P
Merzel, J
Novaes, P D
Institución
Resumen
The frequency of ciliated fibroblasts in skin, gingiva, molar and incisor periodontal ligaments and incisor enamel-related periodontium of the mouse was estimated by straight counting or by methods based on the probability of observing a basal body in relation to other cell structures. Transmission electron microscopy of ultra-thin sections mounted in single slot grids was used. The results obtained with these methods differed, but indicated that periodontal ligament fibroblasts from molars or incisors generally had a higher ciliation index than the fibroblasts from skin and gingiva. These differences may not be real since the detection of cilia and/or centriolar structures seems to depend very much upon the plane of sectioning relative to the long axis of the fibroblasts, a situation which favours the more regularly arranged periodontal fibroblasts. This arrangement makes the periodontal tissues, particularly those of rodent incisors, a valuable model for studying ciliation in vivo because of the prompt response to experimental manipulation. 35 83-9