Escherichia Coli Strains From Edema Disease: O Serogroups, And Genes For Shiga Toxin, Enterotoxins, And F18 Fimbriae.

dc.creatorda Silva, A S
dc.creatorValadares, G F
dc.creatorPenatti, M P
dc.creatorBrito, B G
dc.creatorda Silva Leite, D
dc.date2001-Jun
dc.date2015-11-27T12:29:15Z
dc.date2015-11-27T12:29:15Z
dc.date.accessioned2018-03-29T00:55:56Z
dc.date.available2018-03-29T00:55:56Z
dc.identifierVeterinary Microbiology. v. 80, n. 3, p. 227-33, 2001-Jun.
dc.identifier0378-1135
dc.identifier
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/11337138
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/194993
dc.identifier11337138
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1295226
dc.descriptionThe objectives of the research were to determine the presence of the gene sequences for Shiga Toxin 2e (Stx2e), enterotoxins (ST-I, ST-II and LT-I), and F18 fimbriae in 144 Escherichia coli strains isolated from pigs with edema disease; to assess the ability of stx2e(+) strains to produce Stx2e; and to determine the O serogroups of the E. coli strains. Presence of the genes was determined by polymerase chain reaction (PCR), production of Stx2e was assessed by cytotoxicity for Vero and Hela cells, O serogroups were identified by agglutination with specific antisera. Of the 144 strains tested, 99 were stx2e(+) by PCR, but only 45 of these were Stx2e(+) in the cell culture assays. Among the 99 stx2e(+) strains, PCR detected the genes for F18ab, ST-I, ST-II, LT-I in 76, 40, 31 and 16 strains, respectively. Forty-one of the 99 sxt2e(+) strains belonged to O group 139; the rest did not belong to the classical edema disease O serogroups. It is likely that the enterotoxins, whose genes were detected at high frequency, are responsible for diarrhea seem in pigs with edema disease in Brazil.
dc.description80
dc.description227-33
dc.languageeng
dc.relationVeterinary Microbiology
dc.relationVet. Microbiol.
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectAgglutination Tests
dc.subjectAnimals
dc.subjectBrazil
dc.subjectCercopithecus Aethiops
dc.subjectEdema Disease Of Swine
dc.subjectEscherichia Coli
dc.subjectEscherichia Coli Infections
dc.subjectFimbriae, Bacterial
dc.subjectHela Cells
dc.subjectHumans
dc.subjectO Antigens
dc.subjectPolymerase Chain Reaction
dc.subjectShiga Toxin 2
dc.subjectShiga Toxins
dc.subjectVero Cells
dc.titleEscherichia Coli Strains From Edema Disease: O Serogroups, And Genes For Shiga Toxin, Enterotoxins, And F18 Fimbriae.
dc.typeArtículos de revistas


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