Histoenzymological And Ultrastructural Changes In Lateral Muscle Fibers Of Oreochromis Niloticus (teleostei: Cichlidae) After Local Injection Of Veratrine.

dc.creatorSilva Freitas, M
dc.creatorDal Pai Silva, M
dc.creatorda Cruz-Höfling, A
dc.date2001-Dec
dc.date2015-11-27T12:29:09Z
dc.date2015-11-27T12:29:09Z
dc.date.accessioned2018-03-29T00:55:41Z
dc.date.available2018-03-29T00:55:41Z
dc.identifierHistochemistry And Cell Biology. v. 116, n. 6, p. 525-34, 2001-Dec.
dc.identifier0948-6143
dc.identifier10.1007/s00418-001-0347-1
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/11810194
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/194928
dc.identifier11810194
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1295161
dc.descriptionThe effects of veratrine have been investigated in mammalian, amphibian, and crustacean muscle, but not in fish. In this work, the action of veratrine was studied in the lateral muscle of the freshwater teleost Oreochromis niloticus after intramuscular injection. Histoenzymological typing and electron microscopy of muscle fibers before and 15, 30, and 60 min after veratrine injection (10 ng/kg fish) were used to indirectly assess the morphological changes and the oxidative and m-ATPase activities. In some cases, muscles were pretreated with tetrodotoxin to determine whether the ultrastructural changes were the result of Na(+) channel activation by veratrine. Veratrine altered the metabolism of fibers mainly after 30 min. Oxidative fibers showed decreased NADH-TR activity, whereas that of glycolytic and oxidative-glycolytic type fibers increased. There was no change in the m-ATPase activity of the three fiber types, except at 60 min postveratrine, when a novel fiber type, which showed no reversal after acidic and alkaline preincubations, appeared. Ultrastructural damage involved sarcomeres, myofibrils, and mitochondria, but the T-tubules remained intact. Pretreatment with tetrodotoxin (1 ng/ml) prevented the ultrastructural changes caused by veratrine. These results show that in fish skeletal muscle veratrine produces some effects that are not seen in mammalian muscle.
dc.description116
dc.description525-34
dc.languageeng
dc.relationHistochemistry And Cell Biology
dc.relationHistochem. Cell Biol.
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectAdenosine Triphosphatases
dc.subjectCichlids
dc.subjectDrug Antagonism
dc.subjectInjections, Intramuscular
dc.subjectMicroscopy, Electron
dc.subjectMitochondria, Muscle
dc.subjectMuscle Fibers, Fast-twitch
dc.subjectMuscle Fibers, Slow-twitch
dc.subjectMuscle, Skeletal
dc.subjectSarcomeres
dc.subjectTetrodotoxin
dc.subjectVeratrine
dc.titleHistoenzymological And Ultrastructural Changes In Lateral Muscle Fibers Of Oreochromis Niloticus (teleostei: Cichlidae) After Local Injection Of Veratrine.
dc.typeArtículos de revistas


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