Artículos de revistas
Lack Of Mutations Of Exon 2 Of The Men1 Gene In Endocrine And Nonendocrine Sporadic Tumors.
Registro en:
Brazilian Journal Of Medical And Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas / Sociedade Brasileira De Biofísica ... [et Al.]. v. 34, n. 7, p. 861-5, 2001-Jul.
0100-879X
11449303
Autor
Costa, S C
Nascimento, L S
Ferreira, F J
Mattos, P S
Camara-Lopes, L H
Ward, L S
Institución
Resumen
In addition to the mutations that underlie most cases of the multiple endocrine neoplasia type 1 (MEN1) syndrome, somatic mutations of the MEN1 gene have also been described in sporadic tumors like gastrinomas, insulinomas and bronchial carcinoid neoplasm. We examined exon 2 of this gene, where most of the mutations have been described, in 148 endocrine and nonendocrine sporadic tumors. DNA was obtained by phenol/chloroform extraction and ethanol precipitation from 92 formalin-fixed, paraffin-embedded samples, and from 40 fresh tumor tissue samples. We used 5 pairs of primers to encompass the complete coding sequence of exon 2 of the MEN1 gene that was screened by the polymerase chain reaction-single-stranded conformation polymorphism (PCR-SSCP) technique in 78 sporadic thyroid cancers: 28 follicular adenomas, 35 papillary carcinomas, 14 follicular carcinomas, and 1 anaplastic thyroid carcinoma. We also examined 46 adrenal lesions (3 hyperplasias, 3 adenomas and 35 adrenocortical carcinomas, 2 pheochromocytomas, 2 ganglioneuroblastomas, and 1 lymphoma) and 24 breast cancers (6 noninvasive, 16 infiltrating ductal, and 2 invasive lobular tumors). The PCR product of 5 tumors suspected to present band shifts by SSCP was cloned. Direct sense and antisense sequencing did not identify mutations. These results suggest that the MEN1 gene is not important in breast, thyroid or adrenal sporadic tumorigenesis. Because the frequency of mutations varies significantly among tumor subgroups and allelic deletions are frequently observed at 11q13 in thyroid and adrenal cancers, another tumor suppressor gene residing in this region is likely to be involved in the tumorigenesis of these neoplasms. 34 861-5
Materias
Ítems relacionados
Mostrando ítems relacionados por Título, autor o materia.
-
Cardiorespiratory fitness and site-specific risk of cancer in men: A systematic review and meta-analysis
Pozuelo-Carrascosa D.P.; Alvarez-Bueno C.; Cavero-Redondo I.; Morais S.; Lee I.M.; Martínez-Vizcaíno V. (Elsevier Ltd, 2019) -
El estado del arte en las infecciones producidas por el virus del papiloma humano
Sánchez Vásquez, Gloria Inés; Muñoz, Nubia; Reina, Julio Cesar -
El estado del arte en las infecciones producidas por el virus del papiloma humano.
Reina, Julio César; Muñoz, Nubia; Sánchez, Gloria Inés (2012-12-11)La infección anogenital por el virus del papiloma humano (VPH) es la infección de transmisión sexual mas frecuente en todo el mundo. En los humanos se han identificado alrededor de 100 genotipos de los cuales unos 40 ...