Artículos de revistas
A Quantitative Enzyme-linked Immunosorbent Assay (elisa) For The Immunodiagnosis Of Neurocysticercosis Using A Purified Fraction From Taenia Solium Cysticerci.
Registro en:
Diagnostic Microbiology And Infectious Disease. v. 37, n. 2, p. 87-92, 2000-Jun.
0732-8893
10863102
Autor
da Silva, A D
Quagliato, E M
Rossi, C L
Institución
Resumen
A quantitative enzyme-linked immunosorbent assay (ELISA) for the immunodiagnosis of neurocysticercosis is described. The ELISA was standardized using a purified Taenia solium cysticerci fraction (PCF) obtained by ion exchange chromatography. The ELISA using PCF (PCF-ELISA) and a qualitative ELISA using a whole extract from T. solium cysticerci (WEC-ELISA) were used to screen for cysticercus-specific IgG antibodies in cerebrospinal fluid (CSF) samples from 57 patients with neurocysticercosis and 50 patients with other neurologic disorders. The sensitivity of both assays was 95%, whereas the specificities of PCF-ELISA and WEC-ELISA were 100% and 92%, respectively. The excellent sensitivity and specificity of the PCF-ELISA make this assay a potentially useful tool in screening for antibodies against T. solium cysticerci. 37 87-92