Amino Acid Sequence Of Piratoxin-i, A Myotoxin From Bothrops Pirajai Snake Venom, And Its Biological Activity After Alkylation With P-bromophenacyl Bromide.

dc.creatorToyama, M H
dc.creatorSoares, A M
dc.creatorVieira, C A
dc.creatorNovello, J C
dc.creatorOliveira, B
dc.creatorGiglio, J R
dc.creatorMarangoni, S
dc.date1998-Oct
dc.date2015-11-27T12:19:25Z
dc.date2015-11-27T12:19:25Z
dc.date.accessioned2018-03-29T00:53:09Z
dc.date.available2018-03-29T00:53:09Z
dc.identifierJournal Of Protein Chemistry. v. 17, n. 7, p. 713-8, 1998-Oct.
dc.identifier0277-8033
dc.identifier
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/9853687
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/194267
dc.identifier9853687
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1294500
dc.descriptionThe complete sequence of the 121 amino acid residues of piratoxin-I (PrTX-I), a phospholipase A2 (PLA2)-like myotoxin from Bothrops pirajai snake (Bahia jararacussu) venom, is reported. From the sequence, an M, of 13,825 and an approximate pI of 8.3 were calculated. PrTX-I shows a high sequence homology with Lys-49 myotoxins from other bothropic (approximately 95%) and nonbothropic (approximately 80%) venoms, but only 70-75% homology when aligned with the catalytically active Asp-49 PLA2s. When compared with bothropstoxin-I from Bothropsjararacussu, which is morphologically almost identical to B. pirajai, only two changes out of 121 total amino acid residues have been observed. The approximate minimal lethal dose LD50 (mice, i.p., 24 hr) of PrTX-I was 8 (6.8-9.1) mg/kg, and the minimal edematogenic dose (MED) in a rat paw model was 39.5+/-1.8 ug. After alkylation of His-48 with p-bromophenacyl bromide, the MED was 40.1+/-1.9 ug, but up to 4 LD50 were unable to cause death in any of a group of eight mice after 72 hr. Therefore the edematogenic activity was retained and apparently did not involve His-48, suggesting that at least two biologically active sites are present in PrTX-I.
dc.description17
dc.description713-8
dc.languageeng
dc.relationJournal Of Protein Chemistry
dc.relationJ. Protein Chem.
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectAcetophenones
dc.subjectAlkylation
dc.subjectAmino Acid Sequence
dc.subjectAnimals
dc.subjectChromatography, High Pressure Liquid
dc.subjectCrotalid Venoms
dc.subjectEnzyme Inhibitors
dc.subjectGroup Ii Phospholipases A2
dc.subjectHistidine
dc.subjectLethal Dose 50
dc.subjectMice
dc.subjectMolecular Sequence Data
dc.subjectPhospholipases A
dc.subjectPhospholipases A2
dc.subjectRats
dc.subjectReptilian Proteins
dc.subjectSequence Alignment
dc.subjectSequence Homology, Amino Acid
dc.subjectStructure-activity Relationship
dc.titleAmino Acid Sequence Of Piratoxin-i, A Myotoxin From Bothrops Pirajai Snake Venom, And Its Biological Activity After Alkylation With P-bromophenacyl Bromide.
dc.typeArtículos de revistas


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