Artículos de revistas
Rapid Detection Of Factor V Leiden (fvq506) By Non-radioactive Single Strand Conformation Polymorphism (sscp).
Registro en:
Sangre. v. 41, n. 5, p. 379-81, 1996-Oct.
0036-4355
8986114
Autor
Arruda, V R
von Zuben, P M
Annichino-Bizzachi, J M
Costa, F F
Institución
Resumen
The transition G-->A at position 506 of the factor V gene is responsible for resistance to the anticoagulant effect of activated protein C (APC-resistance), and represents the most common hereditary risk factor for venous thrombosis. A comparison of the ability of non-radioactive single-strand conformation polymorphism (SSCP) and of a standard PCR procedure followed by further digestion with MnlI to detect this factor V gene (FVQ 506) transition indicates that these is a good agreement between the two methods. Non-radioactive SSCP analysis therefore represents a rapid and sensitive alternative for the diagnosis of this important point mutation. 41 379-81