Artículos de revistas
Targeted Development Of Microsatellite Markers From Inter-alu Amplification Of Yac Clones.
Genomics. v. 19, n. 2, p. 391-3, 1994-Jan.
de Souza, A P
Beckmann, J S
Primary genetic maps based on highly informative markers are now available. The local density of these markers may, however, not be sufficient. There is thus a need for new means to generate polymorphic markers from targeted regions of the genome. This can be achieved by selectively cloning and sequencing (CA)n-positive human inter-Alu sequences from targeted YAC clones. This method was tested on 21 YACs and led to the development of seven new polymorphic microsatellite markers.19391-3