Image Analysis Of Feulgen-stained C-h-ras-transformed Nih/3t3 Cells.

dc.creatorMello, M L
dc.creatorRusso, J
dc.date
dc.date2015-11-27T12:18:25Z
dc.date2015-11-27T12:18:25Z
dc.date.accessioned2018-03-29T00:51:07Z
dc.date.available2018-03-29T00:51:07Z
dc.identifierBiochemistry And Cell Biology = Biochimie Et Biologie Cellulaire. v. 68, n. 7-8, p. 1026-31
dc.identifier0829-8211
dc.identifier
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/2223010
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/193740
dc.identifier2223010
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1293973
dc.descriptionFeulgen-DNA content, nuclear phenotypes, and levels of chromatin condensation were evaluated by image analysis in NIH/3T3 cells transformed with the c-H-ras oncogene of T24 cells. Three nuclear phenotypes, differing from those of untransformed control cells and defined in terms of patterns of chromatin condensation, were demonstrated microspectrophotometrically for the tumor cells. Polyploidy could only be observed in nuclei with extensive and deeply stained areas covered with condensed chromatin, i.e., only in a small fraction of the tumor cell nuclear population. The increased chromatin condensation that appeared with cell transformation affected the euchromatin zones. The image analysis provided data that, compared with those obtained in other situations involving cell transformation, could be relevant to the understanding of changes in chromatin supraorganization related to tumorigenesis and to tumor cell diagnosis.
dc.description68
dc.description1026-31
dc.languageeng
dc.relationBiochemistry And Cell Biology = Biochimie Et Biologie Cellulaire
dc.relationBiochem. Cell Biol.
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectAnimals
dc.subjectCell Line, Transformed
dc.subjectCell Nucleus
dc.subjectCell Transformation, Neoplastic
dc.subjectChromatin
dc.subjectColoring Agents
dc.subjectDna, Neoplasm
dc.subjectGenes, Ras
dc.subjectImage Processing, Computer-assisted
dc.subjectMice
dc.subjectMice, Nude
dc.subjectNeoplasm Transplantation
dc.subjectPhenotype
dc.subjectRosaniline Dyes
dc.subjectSpectrophotometry
dc.subjectTransfection
dc.titleImage Analysis Of Feulgen-stained C-h-ras-transformed Nih/3t3 Cells.
dc.typeArtículos de revistas


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