Artículos de revistas
Optimization of enantioselective resolution of racemic ibuprofen by native lipase from Aspergillus niger
Registro en:
Journal Of Industrial Microbiology & Biotechnology. Springer Heidelberg, v. 33, n. 8, n. 713, n. 718, 2006.
1367-5435
WOS:000239677900011
10.1007/s10295-006-0138-8
Autor
Carvalho, PD
Contesini, FJ
Bizaco, R
Calafatti, SA
Macedo, GA
Institución
Resumen
Resolution of (R,S)-ibuprofen (2-(4-isobutylphenyl)propionic acid) enantiomers by esterification reaction with 1-propanol in different organic solvents was studied using native Aspergillus niger lipase. The main variables controlling the process (enzyme concentration and 1-propanol:ibuprofen molar ratio) have been optimized using response surface methodology based on a five-level, two-variable central composite rotatable design, in which the selected objective function was enantioselectivity. This enzyme preparation showed preferentially catalyzes the esterification of R(-)-ibuprofen, and under optimum conditions (7% w/v of enzyme and molar ratio of 2.41:1) the enantiomeric excess of active S(+)-ibuprofen and total conversion values were 79.1 and 48.0%, respectively, and the E-value was 32, after 168 h of reaction in isooctane. 33 8 713 718