Artículos de revistas
Separation and electrochemical detection of paracetamol and 4-aminophenol in a paper-based microfluidic device
Registro en:
Analytica Chimica Acta. Elsevier Science Bv, v. 725, n. 44, n. 50, 2012.
0003-2670
WOS:000303292100005
10.1016/j.aca.2012.03.011
Autor
Shiroma, LY
Santhiago, M
Gobbi, AL
Kubota, LT
Institución
Resumen
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) The present work describes the construction and application of a simple, low cost and sensitive microfluidic paper-based device with electrochemical detection for the detection of paracetamol and 4-aminophenol. The separation channels of a width of 2.0 mm were created on paper using a wax printing process to define the regions of the device. A baseline separation level of the analytes can be obtained in 0.1 mol L-1 acetate buffer solution at pH 4.5 and by injecting 500 nL of the standard solutions at 12 mm from the working electrode. The electrochemical detection system was created at the end of the channels through a process known as sputtering. The previously separated analytes were detected at the end of the hydrophilic separation channel by applying a potential of 400 mV vs. pseudo Au on the working electrode. Experimental variables such as type of paper (cation exchanger and n1), pH, sample volume, applied potential and distance of sample injection were evaluated and, under the conditions of higher response, it was possible to obtain detection limits of 25.0 and 10.0 mu mol L-1 for paracetamol and 4-aminophenol, respectively. (c) 2012 Elsevier B.V. All rights reserved. 725 44 50 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Laboratorio Nacional de Luz Sincrotron (LNLS) Instituto Nacional de Ciencia e Tecnologia de Bioanalitica (INCTBio) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)