Artículos de revistas
A reliable ex vivo invasion assay of human reticulocytes by Plasmodium vivax
Registro en:
Blood. Amer Soc Hematology, v. 118, n. 13, n. E74, n. E81, 2011.
0006-4971
WOS:000295359300002
10.1182/blood-2011-04-348748
Autor
Russell, B
Suwanarusk, R
Borlon, C
Costa, FTM
Chu, CS
Rijken, MJ
Sriprawat, K
Warter, L
Koh, EGL
Malleret, B
Colin, Y
Bertrand, O
Adams, JH
D'Alessandro, U
Snounou, G
Nosten, F
Renia, L
Institución
Resumen
Currently, there are no reliable RBC invasion assays to guide the discovery of vaccines against Plasmodium vivax, the most prevalent malaria parasite in Asia and South America. Here we describe a protocol for an ex vivo P vivax invasion assay that can be easily deployed in laboratories located in endemic countries. The assay is based on mixing enriched cord blood reticulocytes with matured, trypsin-treated P vivax schizonts concentrated from clinical isolates. The reliability of this assay was demonstrated using a large panel of P vivax isolates freshly collected from patients in Thailand. (Blood. 2011;118(13):e74-e81) 118 13 E74 E81 Wellcome Trust of Great Britain, as part of Wellcome Trust-Mahidol University Agency for Science Technology and Research (A*STAR Singapore) Wellcome Trust (United Kingdom) ITM