A reliable ex vivo invasion assay of human reticulocytes by Plasmodium vivax

Russell, B; Suwanarusk, R; Borlon, C; Costa, FTM; Chu, CS; Rijken, MJ; Sriprawat, K; Warter, L; Koh, EGL; Malleret, B; Colin, Y; Bertrand, O; Adams, JH; D'Alessandro, U; Snounou, G; Nosten, F; Renia, L

Artículos de revistas

Registration in:

Blood. Amer Soc Hematology, v. 118, n. 13, n. E74, n. E81, 2011.

0006-4971

WOS:000295359300002

10.1182/blood-2011-04-348748

http://www.repositorio.unicamp.br/jspui/handle/REPOSIP/53691

http://repositorio.unicamp.br/jspui/handle/REPOSIP/53691

http://repositorioslatinoamericanos.uchile.cl/handle/2250/1293279

Author

Russell, B

Suwanarusk, R

Borlon, C

Costa, FTM

Chu, CS

Rijken, MJ

Sriprawat, K

Warter, L

Koh, EGL

Malleret, B

Colin, Y

Bertrand, O

Adams, JH

D'Alessandro, U

Snounou, G

Nosten, F

Renia, L

Institutions

Universidade Estadual de Campinas (Brasil)

Abstract

Currently, there are no reliable RBC invasion assays to guide the discovery of vaccines against Plasmodium vivax, the most prevalent malaria parasite in Asia and South America. Here we describe a protocol for an ex vivo P vivax invasion assay that can be easily deployed in laboratories located in endemic countries. The assay is based on mixing enriched cord blood reticulocytes with matured, trypsin-treated P vivax schizonts concentrated from clinical isolates. The reliability of this assay was demonstrated using a large panel of P vivax isolates freshly collected from patients in Thailand. (Blood. 2011;118(13):e74-e81)

118

E74

E81

Wellcome Trust of Great Britain, as part of Wellcome Trust-Mahidol University

Agency for Science Technology and Research (A*STAR

Singapore)

Wellcome Trust (United Kingdom)

ITM