Artículos de revistas
A reliable ex vivo invasion assay of human reticulocytes by Plasmodium vivax
Blood. Amer Soc Hematology, v. 118, n. 13, n. E74, n. E81, 2011.
Currently, there are no reliable RBC invasion assays to guide the discovery of vaccines against Plasmodium vivax, the most prevalent malaria parasite in Asia and South America. Here we describe a protocol for an ex vivo P vivax invasion assay that can be easily deployed in laboratories located in endemic countries. The assay is based on mixing enriched cord blood reticulocytes with matured, trypsin-treated P vivax schizonts concentrated from clinical isolates. The reliability of this assay was demonstrated using a large panel of P vivax isolates freshly collected from patients in Thailand. (Blood. 2011;118(13):e74-e81)11813E74E81Wellcome Trust of Great Britain, as part of Wellcome Trust-Mahidol UniversityAgency for Science Technology and Research (A*STARSingapore)Wellcome Trust (United Kingdom)ITM