Artículos de revistas
An o-aminobenzoic acid film-based immunoelectrode for detection of the cardiac troponin T in human serum
Registro en:
Biochemical Engineering Journal. Elsevier Science Sa, v. 71, n. 97, n. 104, 2013.
1369-703X
WOS:000315016800013
10.1016/j.bej.2012.12.005
Autor
Mattos, AB
Freitas, TA
Kubota, LT
Dutra, RF
Institución
Resumen
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) An amperometric immunosensor for cardiac troponin T detection in human serum troponin T, a marker considered as "gold standard" for acute myocardial infarction diagnosis, is described. A stable carboxylic film to covalently bind antibodies against cTnT onto electrode surface was achieved with electropolymerization of the o-aminobenzoic acid. A fractional factorial study was performed to optimize the electropolymerization parameters. Cyclic voltammetry assays were carried out for characterize steps of the modified electrode surface. The obtained calibration curve at -0.05 V by amperometry presented a good linear response range from 0.05 to 5.0 ngmL(-1) cTnT with a correlation coefficient of 0.992 (n = 6) and 0.016 ng mL(-1) detection limit. The electrodes showed a good stability upon the analytical responses retaining 91.6% of its initial response after 18 days. This sensor showed outgoing results regarding sensitivity allowing reliable measurements of the cTnT at levels of clinical significance for acute myocardial infarctions diagnosis. (C) 2012 Elsevier B.V. All rights reserved. 71 97 104 Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)