Artículos de revistas
Recovery of extracellular alkaline lipases of fusarium spec. by foam fractionation
Registro en:
Fresenius Environmental Bulletin. Parlar Scientific Publications (p S P), v. 16, n. 11B, n. 1503, n. 1508, 2007.
1018-4619
WOS:000252003500013
Autor
dos Prazeres, JN
Simiqueli, AP
Pastore, GM
Linke, D
Zorn, H
Nimtz, M
Berger, RG
Institución
Resumen
Foam fractionation was used to concentrate and to purify extracellular alkaline lipases produced by Fusarium spec. in submerged cultures supplemented with Tween 80. Gas flow rate and pH-value proved to be crucial process parameters. Maximum recovery rates of lipolytic activity were obtained at 60 mL min(-1) N2 and pH 8.5. Under these conditions, > 90% of the total lipase activity was recovered in the foam with enrichment and purification factors of 8.8 and 5.9. respectively. The secreted lipases were characterized biochemically by means of IEF electrophoresis with activity staining and electrospray tandem mass spectrometry. 16 11B 1503 1508