Tempol Reduces Podocyte Apoptosis via PARP Signaling Pathway in Experimental Diabetes Mellitus

dc.creatorPeixoto, EBMI
dc.creatorPapadimitriou, A
dc.creatorde Faria, JML
dc.creatorde Faria, JBL
dc.date2012
dc.date2014-08-01T18:26:00Z
dc.date2015-11-26T18:02:26Z
dc.date2014-08-01T18:26:00Z
dc.date2015-11-26T18:02:26Z
dc.date.accessioned2018-03-29T00:44:07Z
dc.date.available2018-03-29T00:44:07Z
dc.identifierNephron Experimental Nephrology. Karger, v. 120, n. 2, n. E81, n. E90, 2012.
dc.identifier1660-2129
dc.identifierWOS:000304319200004
dc.identifier10.1159/000337364
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/78844
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/78844
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1292235
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionBackground/Aims: In diabetic hypertensive rats, tempol reduces albuminuria by restoring the redox imbalance. Increased formation of reactive oxygen species leading to activation of poly(ADP-ribose) polymerase (PARP)-1 and podocyte loss by apoptosis contribute to albuminuria in diabetes mellitus (DM). In the present study, we investigated the hypothesis that in DM tempol reduces albuminuria by inhibition of PARP-induced podocyte apoptosis. Methods: DM was induced in 4-week-old spontaneously hypertensive rats by streptozotocin. Mouse and human podocyte cell lines were cultured in normal or high-glucose conditions, with or without tempol and/or a PARP-1 inhibitor, PJ34. Results: In diabetic rats, tempol treatment did not affect plasma glucose levels or systolic blood pressure. Albuminuria was higher in diabetic rats, and it was reduced by tempol. DM leads to an elevation of glomerular apoptotic cells and to podocyte loss; both were prevented by tempol treatment. DM increases the expression of poly(ADP-ribose)-modified proteins in isolated glomeruli, and it was reduced by tempol. In vitro, high glucose increased caspase-3 activity and led to a higher number of apoptotic cells that were prevented by tempol and the PARP-1 inhibitor. Conclusion: In DM, tempol reduces albuminuria associated with reduction of podocyte apoptosis and decreasing oxidative stress via PARP signaling. Copyright (C) 2012 S. Karger AG, Basel
dc.description120
dc.description2
dc.descriptionE81
dc.descriptionE90
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionFAPESP [07/51137-5]
dc.languageen
dc.publisherKarger
dc.publisherBasel
dc.publisherSuíça
dc.relationNephron Experimental Nephrology
dc.relationNephron Exp. Nephrol
dc.rightsfechado
dc.rightshttp://www.karger.com/Services/RightsPermissions
dc.sourceWeb of Science
dc.subjectPodocyte
dc.subjectApoptosis
dc.subjectPoly(ADP-ribose) polymerase
dc.subjectDiabetic nephropathy
dc.subjectAlbuminuria
dc.subjectOxidative stress
dc.subjectTempol
dc.subjectOxidative Stress
dc.subjectNephropathy
dc.subjectHypertension
dc.subjectDisease
dc.subjectInjury
dc.subjectRats
dc.titleTempol Reduces Podocyte Apoptosis via PARP Signaling Pathway in Experimental Diabetes Mellitus
dc.typeArtículos de revistas


Este ítem pertenece a la siguiente institución