Temporal Changes in Matrix Metalloproteinases, Their Inhibitors, and Cathepsins in Mouse Pubic Symphysis During Pregnancy and Postpartum

dc.creatorRosa, RG
dc.creatorTarsitano, CAB
dc.creatorHyslop, S
dc.creatorYamada, AT
dc.creatorToledo, OMS
dc.creatorJoazeiro, PP
dc.date2011
dc.date2014-08-01T18:26:00Z
dc.date2015-11-26T18:02:26Z
dc.date2014-08-01T18:26:00Z
dc.date2015-11-26T18:02:26Z
dc.date.accessioned2018-03-29T00:44:07Z
dc.date.available2018-03-29T00:44:07Z
dc.identifierReproductive Sciences. Sage Publications Inc, v. 18, n. 10, n. 963, n. 977, 2011.
dc.identifier1933-7191
dc.identifierWOS:000295638500005
dc.identifier10.1177/1933719111401657
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/78848
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/78848
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1292233
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionRemodeling and relaxation of the mouse pubic symphysis (PS) are central events in parturition. The involvement of endogenous proteins such as matrix metalloproteinases (MMPs), tissue inhibitors of matrix metalloproteinases (TIMPs), and cathepsins in these phenomena remains unclear. In this work, we used a combination of immunolocalization, protein expression/activity, and relative messenger RNA (mRNA) expression to examine the changes in selected MMPs (-2, -9, and -8), TIMPs (-1 and -2), and cathepsins (B and K) during pregnancy and postpartum in mice. Immunohistochemistry revealed the presence of all of these proteins in the cytoplasm of chondrocytes, fibrochondrocytes, and fibroblast-like cells in the interpubic tissues. Zymography showed increases in the active forms of MMP-2 and -9 primarily on days 15 to 19 of pregnancy. Western blotting showed enhanced expression of MMP-8 on days 12 to 15 of pregnancy, with no changes in cathepsins B and K. Matrix metalloproteinases 2, TIMP-1 and -2, and cathepsin B had significant relative gene expression throughout pregnancy. These findings indicate that during pregnancy and postpartum there are variations in the expression and activity of proteins that may have an important role in remodeling the pubic symphysis during these events.
dc.description18
dc.description10
dc.description963
dc.description977
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionCNPq [141765/2007-0, 304910/2006-6, 477535/2008-9]
dc.descriptionFAPESP [05/51844-8]
dc.languageen
dc.publisherSage Publications Inc
dc.publisherThousand Oaks
dc.publisherEUA
dc.relationReproductive Sciences
dc.relationReprod. Sci.
dc.rightsfechado
dc.rightshttp://www.uk.sagepub.com/aboutus/openaccess.htm
dc.sourceWeb of Science
dc.subjectcathepsin
dc.subjectmatrix metalloproteinase
dc.subjectmice
dc.subjectparturition
dc.subjectpubic symphysis
dc.subjectRelaxin Increases Secretion
dc.subjectTissue Inhibitors
dc.subjectCollagen-metabolism
dc.subjectRipening Process
dc.subjectCervical Growth
dc.subjectGene-expression
dc.subjectUterine
dc.subjectMice
dc.subjectMatrix-metalloproteinase-9
dc.subjectParturition
dc.titleTemporal Changes in Matrix Metalloproteinases, Their Inhibitors, and Cathepsins in Mouse Pubic Symphysis During Pregnancy and Postpartum
dc.typeArtículos de revistas


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