Artículos de revistas
Multiwav calibration for creatinine determination in human serum using the Jaffe reaction
Registro en:
Applied Spectroscopy. Soc Applied Spectroscopy, v. 58, n. 1, n. 54, n. 60, 2004.
0003-7028
WOS:000187950400011
10.1366/000370204322729478
Autor
Guterres, MV
Volpe, POL
Ferreira, MMC
Institución
Resumen
Second-order calibration and multivariate spectroscopic-kinetic measurements in the visible region are proposed to improve the Jaffe method for creatinine assay. Analyses performed on synthetic mixtures containing bilirubin, glucose, and albumin confirm that second-order calibration is useful for creatinine determination in human serum. Quantitative determinations of creatinine with the parallel factor analysis (PARAFAC) and direct trilinear decomposition (TLD) methods were compared. It is shown that both methods can be used for creatinine determination in human serum, with an SEP (squared error of prediction) of 2.22 and coefficient of variability of 6.14% for PARAFAC, and an SEP of 2.38 and coefficient of variability of 6.57 % for TLD. 58 1 54 60