Artículos de revistas
Electroanalytical performance of self-assembled monolayer gold electrode for chloramphenicol determination
Registro en:
Microchimica Acta. Springer Wien, v. 169, n. 41732, n. 345, n. 351, 2010.
0026-3672
WOS:000277940000019
10.1007/s00604-010-0339-8
Autor
Codognoto, L
Winter, E
Doretto, KM
Monteiro, GB
Rath, S
Institución
Resumen
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Monolayers of 2-mercapto-5-methylbenzimidazole (MMB) were prepared on a polycrystalline gold electrode via a self-assembly process to produce a self-assembled monolayer. The resulting electrode was investigated by cyclic voltammetry and electrochemical impedance spectroscopy, and applied to the determination of chloramphenicol (CAP) in a pharmaceutical formulation using flow injection analysis along with amperometric detection. The amperometric cell was operated at -0.75 V (vs Ag/AgCl) at a flow rate of 3 mL min(-1). The method was applied to the determination of CAP in ophthalmic solutions, and its performance was compared to a previously validated HPLC method. The response to CAP is linear in the range from 0.050 to 1.000 A mu mol L(-1) (r = 0.9990), and the limit of detection is 44 A mu mol L(-1). 169 41732 345 351 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)