FAK signalling mediates NF-kappa B activation by mechanical stress in cardiac myocytes

dc.creatorCrosara-Alberto, DP
dc.creatorInoue, RY
dc.creatorCosta, CRC
dc.date2009
dc.dateMAY
dc.date2014-11-18T23:57:21Z
dc.date2015-11-26T17:54:59Z
dc.date2014-11-18T23:57:21Z
dc.date2015-11-26T17:54:59Z
dc.date.accessioned2018-03-29T00:38:44Z
dc.date.available2018-03-29T00:38:44Z
dc.identifierClinica Chimica Acta. Elsevier Science Bv, v. 403, n. 41671, n. 81, n. 86, 2009.
dc.identifier0009-8981
dc.identifierWOS:000266190100013
dc.identifier10.1016/j.cca.2009.01.023
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/66736
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/66736
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/66736
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1290912
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionBackground: Nuclear Factor (NF)-kappa B and Focal Adhesion Kinase (FAK) signalling are implicated in cardiomyocyte hypertrophy. We investigated whether FAK signalling contributed towards NF-kappa B activation by mechanical stress in cardiac myocytes. Methods: Experiments were performed with pressure overload rat left ventricle and isolated cardiac myocytes from adult rats and isolated neonatal rat ventricular myocytes (NRVMs) underwent in vitro stretching. Results: Pressure overload induced NF-kappa B increase in myocardial cell nuclear extracts. Adult rat isolated cardiac myocyte confocal microscopy analysis showed NF-kappa B detected into nuclei. Cyclic stretch increased the amount and NF-kappa B DNA-binding activity in NRVMs nuclear extracts. NRVMs confocal microscopy confirmed NF-kappa B subcelullar relocation in stretched cells. Changes in NF-kappa B subcelular location and DNA-binding activity in stretched NRVMs were paralleled by increased FAK phosphorylation, detected in stretched NRVMs by anti-phosphospecific antibody directed to Tyr397. NRVMs treatment with FAK/Src pharmacological inhibitor attenuated NF-kappa B subcelullar relocation and increased DNA binding activity induced by cyclic stretch in cardiac myocytes. Conclusions: FAK signalling coordinates cardiac myocyte NF-kappa B activation in response to mechanical stress. Further studies are needed to elucidate the influence of this signalling pathway on gene transcription regulation, and cardiac myocyte phenotypic changes in response to mechanical stress. (C) 2009 Elsevier B.V. All rights reserved.
dc.description403
dc.description41671
dc.description81
dc.description86
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFAPESP [00/05137-4, 01/11698-1, 00]
dc.descriptionCNPq [521098/97-1]
dc.languageen
dc.publisherElsevier Science Bv
dc.publisherAmsterdam
dc.publisherHolanda
dc.relationClinica Chimica Acta
dc.relationClin. Chim. Acta
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectCardiac myocytes
dc.subjectHeart
dc.subjectHypertrophy
dc.subjectMechanical stress
dc.subjectCell signalling
dc.subjectCyclic stretch
dc.subjectCell stress
dc.subjectFocal adhesion kinase
dc.subjectFocal Adhesion Kinase
dc.subjectHypertrophic Response
dc.subjectTranscription Factor
dc.subjectPressure-overload
dc.subjectCyclic Stretch
dc.subjectRat-heart
dc.subjectC-jun
dc.subjectApoptosis
dc.subjectPathways
dc.subjectReceptor
dc.titleFAK signalling mediates NF-kappa B activation by mechanical stress in cardiac myocytes
dc.typeArtículos de revistas


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