Tissue microarray is a reliable method for immunohistochemical analysis of pleomorphic adenoma

dc.creatorFonseca, FP
dc.creatorde Andrade, BAB
dc.creatorRangel, ALCA
dc.creatorDella Coletta, R
dc.creatorLopes, MA
dc.creatorde Almeida, OP
dc.creatorVargas, PA
dc.date2014
dc.dateJAN
dc.date2014-08-01T18:22:23Z
dc.date2015-11-26T17:54:55Z
dc.date2014-08-01T18:22:23Z
dc.date2015-11-26T17:54:55Z
dc.date.accessioned2018-03-29T00:38:38Z
dc.date.available2018-03-29T00:38:38Z
dc.identifierOral Surgery Oral Medicine Oral Pathology Oral Radiology. Elsevier Science Inc, v. 117, n. 1, n. 81, n. 88, 2014.
dc.identifier2212-4403
dc.identifier1528-395X
dc.identifierWOS:000328633800025
dc.identifier10.1016/j.oooo.2013.08.029
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/77948
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/77948
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1290890
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionObjective. To determine the most adequate number and size of tissue microarray (TMA) cores for pleomorphic adenoma immunohistochemical studies. Study design. Eighty-two pleomorphic adenoma cases were distributed in 3 TMA blocks assembled in triplicate containing 1.0-, 2.0-, and 3.0-mm cores. Immunohistochemical analysis against cytokeratin 7, Ki67, p63, and CD34 were performed and subsequently evaluated with PixelCount, nuclear, and microvessel software applications. Results. The 1.0-mm TMA presented lower results than 2.0-and 3.0-mm TMAs versus conventional whole section slides. Possibly because of an increased amount of stromal tissue, 3.0-mm cores presented a higher microvessel density. Comparing the results obtained with one, two, and three 2.0-mm cores, there was no difference between triplicate or duplicate TMAs and a single-core TMA. Conclusions. Considering the possible loss of cylinders during immunohistochemical reactions, 2.0-mm TMAs in duplicate are a more reliable approach for pleomorphic adenoma immunohistochemical study.
dc.description117
dc.description1
dc.description81
dc.description88
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionFAPESP [2009/53839-2, 2012/07519-9, 2012/10781-7]
dc.languageen
dc.publisherElsevier Science Inc
dc.publisherNew York
dc.publisherEUA
dc.relationOral Surgery Oral Medicine Oral Pathology Oral Radiology
dc.relationOral Surg. Oral Med. Oral Pathol. Oral Radiol.
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectSalivary-glands
dc.subjectValidation
dc.subjectCancer
dc.subjectTechnology
dc.subjectCarcinoma
dc.subjectExpression
dc.subjectTumors
dc.titleTissue microarray is a reliable method for immunohistochemical analysis of pleomorphic adenoma
dc.typeArtículos de revistas


Este ítem pertenece a la siguiente institución