Artículos de revistas
Tissue microarray is a reliable method for immunohistochemical analysis of pleomorphic adenoma
Registro en:
Oral Surgery Oral Medicine Oral Pathology Oral Radiology. Elsevier Science Inc, v. 117, n. 1, n. 81, n. 88, 2014.
2212-4403
1528-395X
WOS:000328633800025
10.1016/j.oooo.2013.08.029
Autor
Fonseca, FP
de Andrade, BAB
Rangel, ALCA
Della Coletta, R
Lopes, MA
de Almeida, OP
Vargas, PA
Institución
Resumen
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Objective. To determine the most adequate number and size of tissue microarray (TMA) cores for pleomorphic adenoma immunohistochemical studies. Study design. Eighty-two pleomorphic adenoma cases were distributed in 3 TMA blocks assembled in triplicate containing 1.0-, 2.0-, and 3.0-mm cores. Immunohistochemical analysis against cytokeratin 7, Ki67, p63, and CD34 were performed and subsequently evaluated with PixelCount, nuclear, and microvessel software applications. Results. The 1.0-mm TMA presented lower results than 2.0-and 3.0-mm TMAs versus conventional whole section slides. Possibly because of an increased amount of stromal tissue, 3.0-mm cores presented a higher microvessel density. Comparing the results obtained with one, two, and three 2.0-mm cores, there was no difference between triplicate or duplicate TMAs and a single-core TMA. Conclusions. Considering the possible loss of cylinders during immunohistochemical reactions, 2.0-mm TMAs in duplicate are a more reliable approach for pleomorphic adenoma immunohistochemical study. 117 1 81 88 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) FAPESP [2009/53839-2, 2012/07519-9, 2012/10781-7]