Standardization of Histological Procedures for the Detection of Toxic Substances by Immunohistochemistry in Dipteran Larvae of Forensic Importance

dc.creatorSouza, CM
dc.creatorLima, CGP
dc.creatorAlves, MJ
dc.creatorArrais-Silva, WW
dc.creatorGiorgio, S
dc.creatorLinhares, AX
dc.creatorThyssen, PJ
dc.date2013
dc.dateJUL
dc.date2014-07-30T20:02:42Z
dc.date2015-11-26T17:52:46Z
dc.date2014-07-30T20:02:42Z
dc.date2015-11-26T17:52:46Z
dc.date.accessioned2018-03-29T00:36:18Z
dc.date.available2018-03-29T00:36:18Z
dc.identifierJournal Of Forensic Sciences. Wiley-blackwell, v. 58, n. 4, n. 1015, n. 1021, 2013.
dc.identifier0022-1198
dc.identifierWOS:000321491200027
dc.identifier10.1111/1556-4029.12140
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/74537
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/74537
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1290349
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionImmunohistochemistry (IHC) technique is an alternative toxicological analysis to detect drugs in insects of forensic importance, but it requires thorough histological procedures. In this study, we tested different fixatives-phosphate-buffered paraformaldehyde 4% (PP), Carnoy's fluid (CF), Kahle's solution (KS), ethanol in different concentrations, and ethanol associated to PP and CF, time of fixation and histological processes for dipteran larvae's tissue, aiming to develop a sample preparation protocol for IHC application. A suitable fixation was achieved using PP for 12 and 24 h, CF for 3 h, 70% ethanol for 19 days, and 70% ethanol/CF for 2 h/3 h. Postfixation using negative pressure, two immersions in xylene for 30 min each, and one in xylene plus paraffin for 45 min increased tissue preservation. An immunohistochemical test for cocaine detection was performed using monoclonal benzoylecgonine antibody from mouse, peroxidase-conjugated anti-mouse IgG and visualized by 3,3'-diaminobenzidine method showed these histological procedures didn't compromise antigenicity.
dc.description58
dc.description4
dc.description1015
dc.description1021
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionFAPESP [2004/08544-0, 2005/54480-7, 2006/50564-4]
dc.languageen
dc.publisherWiley-blackwell
dc.publisherHoboken
dc.publisherEUA
dc.relationJournal Of Forensic Sciences
dc.relationJ. Forensic Sci.
dc.rightsfechado
dc.rightshttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dc.sourceWeb of Science
dc.subjectforensic science
dc.subjectforensic entomology
dc.subjectentomotoxicology
dc.subjecthistological technique
dc.subjectimmunohistochemical analyses
dc.subjectfixatives
dc.subjectParasarcophaga-ruficornis Diptera
dc.subjectCalliphora-vicina Diptera
dc.subjectDecomposing Tissues
dc.subjectDrug Levels
dc.subjectPostmortem Intervals
dc.subjectRabbit Carcasses
dc.subjectMusca-domestica
dc.subjectFly Larvae
dc.subjectMorphine
dc.subjectSarcophagidae
dc.titleStandardization of Histological Procedures for the Detection of Toxic Substances by Immunohistochemistry in Dipteran Larvae of Forensic Importance
dc.typeArtículos de revistas


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