PROSTAGLANDIN E(2), REGULATES INDUCIBLE NITRIC-OXIDE SYNTHASE IN THE MURINE MACROPHAGE CELL-LINE J774

dc.creatorMILANO, S
dc.creatorARCOLEO, F
dc.creatorDIELI, M
dc.creatorDAGOSTINO, R
dc.creatorDAGOSTINO, P
dc.creatorDENUCCI, G
dc.creatorCILLARI, E
dc.date1995
dc.dateFEB
dc.date2014-12-16T11:32:39Z
dc.date2015-11-26T17:51:15Z
dc.date2014-12-16T11:32:39Z
dc.date2015-11-26T17:51:15Z
dc.date.accessioned2018-03-29T00:34:38Z
dc.date.available2018-03-29T00:34:38Z
dc.identifierProstaglandins & Other Lipid Mediators. Elsevier Science Inc, v. 49, n. 2, n. 105, n. 115, 1995.
dc.identifier1098-8823
dc.identifierWOS:A1995QW92200004
dc.identifier10.1016/0090-6980(94)00004-G
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/58279
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/58279
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/58279
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1289930
dc.descriptionWe have evaluated the role of prostaglandin E(2) (PGE(2)) in the synthesis of nitric oxide (NO) by the activation of the inducible form of nitric oxide synthase (NOS) in the murine macrophage cell line, J774, stimulated with different doses of lipopolysaccharide (LPS). The stimulation of the J774 line with suboptimal doses of LPS (0.1 mu g/mL) caused a production of endogenous PGE(2) that was capable of stimulating NOS activity inducing an increase in the NO synthesis, as attested by the fact that cyclooxygenase enzyme inhibitor, indomethacin, significantly reduced NO secretion. On the contrary, a higher dose of LPS (I mu g/mL) produced high levels of PGE(2) that reduced the levels of NOS and, subsequently, NO production. Experiments carried out with exogenous PGE(2) indicated that concentrations between 1 and 10 ng/mL are able to stimulate the expression of NOS and the release of NO, while higher concentrations (>50 ng/mL) are inhibitory. Furthermore, our data indicate that there is a network of interaction which involves NO, PGE(2), and tumor necrosis factor. High levels of PGE(2) inhibited TNF alpha secretion, which in turn could exert inhibitory effects on NO synthesis.
dc.description49
dc.description2
dc.description105
dc.description115
dc.languageen
dc.publisherElsevier Science Inc
dc.publisherNew York
dc.publisherEUA
dc.relationProstaglandins & Other Lipid Mediators
dc.relationProstaglandins Other Lipid Mediat.
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectCYCLOOXYGENASE ENZYME
dc.subjectINDOMETHACIN
dc.subjectNITRIC OXIDE
dc.subjectNITRIC OXIDE SYNTHASE
dc.subjectPROSTAGLANDIN E(2)
dc.subjectPROSTAGLANDINS
dc.subjectTUMOR NECROSIS FACTOR ALPHA
dc.subjectTumor-necrosis-factor
dc.subjectAortic Endothelial-cells
dc.subjectL-arginine
dc.subjectGene-expression
dc.subjectCyclooxygenase
dc.subjectRelease
dc.subjectLipopolysaccharide
dc.subjectLeishmaniasis
dc.subjectEicosanoids
dc.subjectOxidation
dc.titlePROSTAGLANDIN E(2), REGULATES INDUCIBLE NITRIC-OXIDE SYNTHASE IN THE MURINE MACROPHAGE CELL-LINE J774
dc.typeArtículos de revistas


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