Artículos de revistas
Use of solid-phase extraction, high-performance liquid chromatography, and MALDI-TOF identification for [D-Leu(1)]MCYST-LR analysis in treated water: Validation of the analytical methodology
Registro en:
Canadian Journal Of Analytical Sciences And Spectroscopy. Canadian Soc Analytical Sciences & Spectroscopy, v. 52, n. 1, n. 1, n. 10, 2007.
1205-6685
WOS:000260089400001
Autor
de Albuquerque, EC
Melo, LFC
Franco, TT
Institución
Resumen
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) A reverse-phase high-performance liquid chromatographic (RP-HPLC) method with an ultraviolet (UV) detector for analysis of microcystins (MCYSTs) from treated water was validated. The isocratic system used a C 18 column and mobile phase containing acetonitrile: water (35:65 v/v). A MALDI-TOF spectrometry analysis of Microcystis aeruginosa culture extract showed [ADMAdda5] MCYST-LR, [D-Leu1] MCYST-LR, [D-Asp3, ADMAdda5, Dhb7] MCYST-HtyR, and [D-Asp3, ADMAdda5, Dhb7] MCYST-RR. Linearity, calibration, accuracy, precision, and limits of detection (LOD) and of quanti. cation (LOQ) were investigated. The following parameters were found in treated water samples: a linearity between 0.03 mu g/mL and 2.00 mu g/mL; limits of detection (LOD) and of quanti. cation (LOQ) of 0.05 mu g/mL and 0.15 mu g/mL, respectively; an extraction effciency above 81.09%; and appropriate precisions (on a single day: R.S.D. (n = 3) <4.91% and from one day to the next: R. S. D (n = 3) > 6.79 %) at the different concentrations of [D-Leu1] MCYST-LR. The validated method can be used for determination of MCYST in water within the international limits of 1.0 mu g/L with a 500-fold pre-concentration. 52 1 1 10 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) University of Potsdam, Germany MALDI-TOF Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) FAPESP [0211529-8]