Artículos de revistas
Evidence for the association of the human regulatory protein Ki-1/57 with the translational machinery
Registro en:
Febs Letters. Elsevier Science Bv, v. 585, n. 16, n. 2556, n. 2560, 2011.
0014-5793
WOS:000293826300002
10.1016/j.febslet.2011.07.010
Autor
Goncalves, KD
Bressan, GC
Saito, A
Morello, LG
Zanchin, NIT
Kobarg, J
Institución
Resumen
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Ki-1/57 is a cytoplasmic and nuclear protein of 57 kDa first identified in malignant cells from Hodgkin's lymphoma. Based on yeast-two hybrid protein interaction we found out that Ki-1/57 interacts with adaptor protein RACK1 (receptor of activated kinase 1), CIRP (cold-inducible RNA-binding protein), RPL38 (ribosomal protein L38) and FXR1 (fragile X mental retardation-related protein 1). Since these proteins are involved in the regulation of translation we suspected that Ki-1/57 may have a role in it. We show by immunoprecipitation the association of Ki-1/57 with FMRP. Confocal microscopy revealed that Ki-1/57 colocalizes with FMRP/FXR1/2 to stress granules. Furthermore Ki-1/57 cosediments with free ribosomal particles and enhances translation, when tethered to a reporter mRNA, suggesting that Ki-1/57 may be involved in translational regulation. Structured summary of protein interactions: Ki-1/57 and TIA-1 colocalize by fluorescence microscopy (View interaction) Ki-1/57 physically interacts with CIRP by two hybrid (View interaction) FMRP physically interacts with Ki-1/57 by anti bait coimmunoprecipitation (View interaction) Ki-1/57 physically interacts with FXR1 by two hybrid (View interaction) Ki-1/57 physically interacts with RPL38 by two hybrid (View interaction) (C) 2011 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved. 585 16 2556 2560 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) LNBio/CNPEM Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
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