Differential seminal plasma proteome according to semen retrieval in men with spinal cord injury

dc.creatorda Silva, BF
dc.creatorSouza, GHMF
dc.creatorlo Turco, EG
dc.creatorDel Giudice, PT
dc.creatorSoler, TB
dc.creatorSpaine, DM
dc.creatorBorrelli, M
dc.creatorGozzo, FC
dc.creatorPilau, EJ
dc.creatorGarcia, JS
dc.creatorFerreira, CR
dc.creatorEberlin, MN
dc.creatorBertolla, RP
dc.date2013
dc.dateOCT
dc.date2014-07-30T14:43:35Z
dc.date2015-11-26T17:39:56Z
dc.date2014-07-30T14:43:35Z
dc.date2015-11-26T17:39:56Z
dc.date.accessioned2018-03-29T00:21:34Z
dc.date.available2018-03-29T00:21:34Z
dc.identifierFertility And Sterility. Elsevier Science Inc, v. 100, n. 4, n. 959, n. +, 2013.
dc.identifier0015-0282
dc.identifierWOS:000324995200029
dc.identifier10.1016/j.fertnstert.2013.06.009
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/61817
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/61817
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1286586
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionObjective: To evaluate protein expression profile and to quantify proteins present in seminal plasma from men with spinal cord injury (SCI) and healthy men without SCI. Design: Experimental study. Setting: University hospital. Patient(s): Twelve SCI patients divided into two groups, six who underwent electroejaculation (EEJ) and six who underwent penile vibratory stimulation (PVS); and ten control subjects presenting normal sperm motility and concentration. Intervention(s): EEJ and PVS. Main Outcome Measure(s): The seminal plasma protein profile was analyzed by two proteomic strategies: data-independent label-free quantitative proteomics (MSE) and two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis (2D SDS-PAGE). Result(s): A total of 638 different proteins were identified by MSE and 18 by 2D SDS-PAGE followed by tandem mass spectrometry. Interactome analysis showed key reproductive biologic processes-insemination, sperm and oocyte fusion, and acrosome reaction-related to all groups, as were triglyceride stimuli. Processes related to actin and muscle function and to iron oxidation, transportation, and homeostasis were found only in the EEJ and PVS groups; response to hydrogen peroxide and increased immune response was found only in the PVS group. Conclusion(s): This study was able to demonstrate differential protein expression among control, PVS, and EEJ groups; SCI is responsible for alterations in seminal plasma protein profile leading to a deviation from homeostasis; proteins reported in both PVS and EEJ groups correlate with the pathophysiology of SCI-related infertility. (Fertil Steril (R) 2013; 100: 959-69. (C) 2013 by American Society for Reproductive Medicine.)
dc.description100
dc.description4
dc.description959
dc.description+
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.languageen
dc.publisherElsevier Science Inc
dc.publisherNew York
dc.publisherEUA
dc.relationFertility And Sterility
dc.relationFertil. Steril.
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectBiomarkers
dc.subjectspinal cord injury
dc.subjectseminal plasma
dc.subjectproteomics
dc.subjectfunctional enrichment
dc.subjectProstaglandin D-synthase
dc.subjectSperm Motility
dc.subjectVibratory Stimulation
dc.subjectElectric-current
dc.subjectProstate
dc.subjectElectroejaculation
dc.subjectIdentification
dc.subjectSpermatozoa
dc.subjectInfertility
dc.subjectMechanisms
dc.titleDifferential seminal plasma proteome according to semen retrieval in men with spinal cord injury
dc.typeArtículos de revistas


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