Artículos de revistas
Isolation and characterization of isolectins from Talisia esculenta seeds
Registro en:
Journal Of Protein Chemistry. Kluwer Academic/plenum Publ, v. 20, n. 6, n. 495, n. 500, 2001.
0277-8033
WOS:000172269800007
10.1023/A:1012510614429
Autor
Freire, MDM
Machado, OLT
Smolka, MB
Marangoni, S
Novello, JC
Macedo, MLR
Institución
Resumen
Four isolectins (TEL-I, TEL-II, TEL-III and TEL-IV ) were isolated from seeds of Talisia esculenta by reverse-phase high-performance liquid chromatography. RP-HPLC was performed on a u-Bondapack C18 column (0.78 cm x 30 cm) (Waters 991-PDA system) at room temperature. Rechromatography of the four fractions on a C18 column under the same conditions yielded lectins with two dissimilar subunits (M-r 20 kDa and 40 kDa) bound noncovalently. The isolectins showed very similar characteristics, such as molecular masses, N-terminal sequences, and hemagglutinating activity, but differed in their isoelectric points and in inhibition by carbohydrates. 20 6 495 500