Artículos de revistas
Alginate production by Pseudomonas mendocina in a stirred draft fermenter
Registration in:
World Journal Of Microbiology & Biotechnology. Springer, v. 23, n. 5, n. 691, n. 695, 2007.
0959-3993
WOS:000246206400012
10.1007/s11274-006-9285-3
Author
Muller, JM
Alegre, RM
Institutions
Abstract
In this study alginate production by Pseudomonas mendocina in a laboratory-scale fermenter was investigated. In the experiments the effect of temperature (25-31 degrees C) and agitation (500-620 rev min(-1)) at a constant air flow of 10 v/v/h were evaluated in relation to the rate of glucose bioconversion to alginate using response surface methodology (RSM). The fermenter configuration was also adapted to a system with a screw mixer and draft tube, due to the change in rheological characteristics of the fermentation broth. The adjusted model indicates a temperature of 29.1 degrees C and agitation of 553 rev min(-1) for optimum alginate synthesis. In this fermentation system a Y-p/s of 44.8% was achieved. The alginate synthesized by P. mendocina showed a partially acetylated pattern as previously reported for alginates obtained from other Pseudomonas spp and Azotobacter vinelandii. 23 5 691 695