Artículos de revistas
Detection of human herpesvirus-7 by qualitative nested-PCR: comparison between healthy individuals and liver transplant recipients
Registro en:
Revista Da Sociedade Brasileira De Medicina Tropical. Soc Brasileira Medicina Tropical, v. 41, n. 6, n. 556, n. 559, 2008.
0037-8682
WOS:000262440500002
Autor
Thomasini, RL
Martins, JD
Parola, DC
Bonon, SHA
Boin, IDSF
Leonardi, LS
Leonardi, M
Costa, SCB
Institución
Resumen
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Diagnosis of human herpesvirus-7 active infection in transplant patients has proved difficult, because this virus is ubiquitous and can cause persistent infections in the host. The significance of viral DNA detected in leukocytes by PCR is unclear and cross-reaction in serological tests may occur. This study aimed to evaluate nested-PCR to detect human herpesvirus-7 active infection in liver transplant recipients compared to healthy individuals. human herpesvirus-7 nested-PCR was performed on leukocytes and sera of 53 healthy volunteers and sera of 29 liver transplant recipients. In healthy volunteers, human herpesvirus-7 was detected in 28.3% of leukocytes and 0% of serum. human herpesvirus-7 was detected in sera of 48.2% of the liver transplant recipients. Nested-PCR on DNA extracted from leukocytes detected latent infection and the study suggests that nested-PCR performed on serum could be useful to detect human herpesvirus-7 active infection in liver transplant recipients. 41 6 556 559 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Herminio Ometto Foundation Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)