Artículos de revistas
Partitioning of pectinolytic enzymes in polyethylene glycol/potassium phosphate aqueous two-phase systems
Registro en:
Carbohydrate Polymers. Elsevier Sci Ltd, v. 50, n. 1, n. 63, n. 68, 2002.
0144-8617
WOS:000176502800009
10.1016/S0144-8617(01)00376-9
Autor
Lima, AS
Alegre, RM
Meirelles, AJA
Institución
Resumen
Four different pectinolytic enzymes have been partitioned in polyethylene glycol (PEG)-potassium phosphate aqueous two-phase systems (20-15%). The influence of PEG molecular weight and the addition of sodium chloride were investigated. The best results concerning to the partition coefficients (K-p and K-e) and the yield recovery (R) were: PEG-400/phosphate with NaCl for exo-polygalacturonase (K-e = 5.35 and R = 89.5%), PEG-600/phosphate for pectin lyase (PL) (K-e = 43.18 and R = 98.5%) and pectinesterase (PE) (K-e = 1.51 and R 69.6%), and PEG-10,000/phosphate for endo-polygalacturonase (K-e = 1.35 and R = 53.5%). The best purification factors were observed in the upper phase for the systems containing high molecular weight PEG without NaCl: PEG-6000 for exo-polygalacturonase (5.49 fold) and PEG-10,000 for endo-polygalacturonase (16.28 fold), PE (16.64 fold) and PL (14.27 fold). (C) 2002 Elsevier Science Ltd. All rights reserved. 50 1 63 68