Mitochondrial effects of triarylmethane dyes

dc.creatorKowaltowski, AJ
dc.creatorTurin, J
dc.creatorIndig, GL
dc.creatorVercesi, AE
dc.date1999
dc.dateDEC
dc.date2014-12-02T16:25:38Z
dc.date2015-11-26T17:22:48Z
dc.date2014-12-02T16:25:38Z
dc.date2015-11-26T17:22:48Z
dc.date.accessioned2018-03-29T00:10:11Z
dc.date.available2018-03-29T00:10:11Z
dc.identifierJournal Of Bioenergetics And Biomembranes. Kluwer Academic/plenum Publ, v. 31, n. 6, n. 581, n. 590, 1999.
dc.identifier0145-479X
dc.identifierWOS:000085188600006
dc.identifier10.1023/A:1005421112345
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/57120
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/57120
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/57120
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1283680
dc.descriptionThe mitochondrial effects of submicromolar concentrations of six triarylmethane dyes, with potential applications in antioncotic photodynamic therapy, were studied. All dyes promoted an inhibition of glutamate or succinate-supported respiration in uncoupled mitochondria, in a manner stimulated photodynamically. No inhibition of N,N,N',N'-tetramethyl-p-phenylenediamine (TMPD) supported respiration was observed, indicating that these dyes do not affect mitochondrial complex IV. When mitochondria were energized with TMPD in the absence of an uncoupler, treatment with victoria blue R, B, or BO, promoted a dissipation of mitochondrial membrane potential and increase of respiratory rates, compatible with mitochondrial uncoupling. This effect was observed even in the dark, and was not prevented by EGTA, Mg2+ or cyclosporin A, suggesting that it is promoted by a direct effect of the dye on inner mitochondrial membrane permeability to protons. Indeed, victoria blue R, B, and BO promoted swelling of valinomycin-treated mitochondria incubated in a hyposmotic K+-acetate-based medium, confirming that these dyes act as classic protonophores such as FCCP. On the other hand, ethyl violet, crystal violet, and malachite green promoted a dissipation of mitochondrial membrane potential, accompanied by mitochondrial swelling, which was prevented by EGTA, Mg2+, and cyclosporin A, demonstrating that these drugs induce mitochondrial permeability transition. This mitochondrial permeabilization was followed by respiratory inhibition, attributable to cytochrome c release, and was caused by the oxidation of NAD(P)H promoted by these drugs.
dc.description31
dc.description6
dc.description581
dc.description590
dc.languageen
dc.publisherKluwer Academic/plenum Publ
dc.publisherNew York
dc.publisherEUA
dc.relationJournal Of Bioenergetics And Biomembranes
dc.relationJ. Bioenerg. Biomembr.
dc.rightsfechado
dc.sourceWeb of Science
dc.subjectmitochondria
dc.subjecttriarylmethane dyes
dc.subjectphotodynamic therapy
dc.subjectrespiration
dc.subjectmitochondrial permeability transition
dc.subjectcyclosporin A
dc.subjectcalcium
dc.subjectproton transport
dc.subjectVictoria-blue Bo
dc.subjectOxidative-phosphorylation
dc.subjectPermeability Transition
dc.subjectPhotodynamic Therapy
dc.subjectCrystal Violet
dc.subjectMechanism
dc.subjectApoptosis
dc.subjectMembrane
dc.subjectCells
dc.titleMitochondrial effects of triarylmethane dyes
dc.typeArtículos de revistas


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