Biological and enzymatic activities of Micrurus sp (Coral) snake venoms

dc.creatorCecchini, AL
dc.creatorMarcussi, S
dc.creatorSilveira, LB
dc.creatorBorja-Oliveira, CR
dc.creatorRodrigues-Simioni, L
dc.creatorAmara, S
dc.creatorStabeli, RG
dc.creatorGiglio, JR
dc.creatorArantes, EC
dc.creatorSoares, AM
dc.date2005
dc.dateJAN
dc.date2014-11-16T00:58:23Z
dc.date2015-11-26T17:22:38Z
dc.date2014-11-16T00:58:23Z
dc.date2015-11-26T17:22:38Z
dc.date.accessioned2018-03-29T00:10:03Z
dc.date.available2018-03-29T00:10:03Z
dc.identifierComparative Biochemistry And Physiology A-molecular & Integrative Physiology. Elsevier Science Inc, v. 140, n. 1, n. 125, n. 134, 2005.
dc.identifier1095-6433
dc.identifierWOS:000226964400014
dc.identifier10.1016/j.cbpb.2004.11.012
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/55592
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/55592
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/55592
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1283649
dc.descriptionThe venoms of Micrurus lemniscatus carvalhoi, Micrurus frontalis frontalis, Micrurus surinamensis surinamensis and Micrurus nigrocinctus nigrocinctus were assayed for biological activities. Although showing similar liposome disrupting and myotoxic activities, M. frontalis frontalis and M. nigrocinctus nigrocinctus displayed higher anticoagulant and phospholipase A(2) (PLA(2)) activities. The latter induced a higher edema response within 30 min. Both venoms were the most toxic as well. In the isolated chick biventer cervicis preparation, M. lemniscatus carvalhoi venom blocked the indirectly elicited twitch-tension response (85+/-0.6% inhibition after a 15 min incubation at 5 mug of venom/mL) and the response to acetylcholine (ACh; 55 or 110 muM), without affecting the response to KCl (13.4 mM). In mouse phrenic nerve-diaphragm preparation, the venom (5 mug/mL) produced a complete inhibition of the indirectly elicited contractile response after 50 min incubation and did not affect the contractions elicited by direct stimulation. M. lemniscatus carvalhoi inhibited H-3-L-glutamate uptake in brain synaptosomes in a Ca2+, but not time dependent manner. The replacement of Ca2+ by Sr2+ and ethylene glycol-bis(beta-aminoethyl ether) (EGTA), or alkylation of the venom with p-bromophenacyl bromide (BPB), inhibited 3 H-L-glutamate uptake. M lemniscatus carvalhoi venom cross-reacted with postsynaptic alpha-neurotoxins short-chain (antineurotoxin-II) and long-chain (antibungarotoxin) antibodies. It also cross-reacted with antimyotoxic PLA(2) antibodies from M. nigrocinctus nigrocinctus (antinigroxin). Our results point to the need of catalytic activity for these venoms to exert their neurotoxic activity efficiently and to their components as attractive tools for the study of molecular targets on cell membranes. (C) 2004 Elsevier Inc. All rights reserved.
dc.description140
dc.description1
dc.description125
dc.description134
dc.languageen
dc.publisherElsevier Science Inc
dc.publisherNew York
dc.publisherEUA
dc.relationComparative Biochemistry And Physiology A-molecular & Integrative Physiology
dc.relationComp. Biochem. Physiol. A-Mol. Integr. Physiol.
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectcoral snake venoms
dc.subjectMicrurus sp.
dc.subjectMicrurus lemniscatus carvalhoi
dc.subjectliposome-disrupting activity
dc.subjectmyotoxicity
dc.subjectneurotoxicity
dc.subjectphospholipase A(2)
dc.subjectsynaptosome
dc.subjectL-glutamate uptake
dc.subjectPhospholipase A(2) Homolog
dc.subjectFunctional-characterization
dc.subjectPostsynaptic Neurotoxins
dc.subjectNigrocinctus Venom
dc.subjectBeta-bungarotoxin
dc.subjectIn-vitro
dc.subjectGlutamate
dc.subjectAcid
dc.subjectMyonecrosis
dc.subjectAnticoagulant
dc.titleBiological and enzymatic activities of Micrurus sp (Coral) snake venoms
dc.typeArtículos de revistas


Este ítem pertenece a la siguiente institución