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Crystallization of porcine insulin with carbon dioxide as acidifying agent
Registro en:
Powder Technology. Elsevier Science Sa, v. 197, n. 41671, n. 54, n. 57, 2010.
0032-5910
WOS:000272125000007
10.1016/j.powtec.2009.08.017
Autor
Hirata, GAM
Bernardo, A
Miranda, EA
Institución
Resumen
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Recent studies on the use of volatile electrolytes such as CO(2) in protein precipitation showed that these agents are a promising alternative to the conventional acids. This use of volatile electrolytes prevents protein denaturation due to local pH extremes, and saline effluent generation is greatly reduced, as the volatile electrolyte may be separated and recovered from solution just by pressure release. In this work, insulin was successfully crystallized in the presence of zinc using CO(2) as acidifying agent. The crystals obtained were rhombohedral, a common shape for porcine insulin crystals that contain zinc in their structure, and their average size varied with the mixing applied. (C) 2009 Elsevier B.V. All rights reserved 197 41671 54 57 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) (Departamento de Engenharia Quimica da Universidade de Sao Paulo, Brazil) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)