Artículos de revistas
SHP-2 regulates myogenesis by coupling to FAK signaling pathway
Registro en:
Febs Letters. Elsevier Science Bv, v. 583, n. 18, n. 2975, n. 2981, 2009.
0014-5793
WOS:000271284200001
10.1016/j.febslet.2009.08.022
Autor
de Oliveira, MV
Marin, TM
Clemente, CF
Dalla Costa, AP
Judice, CC
Franchini, KG
Institución
Resumen
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Transient dephosphorylation of FAK at Tyr-397 is required for cell cycle withdrawal early on during myogenesis. Here, we show that upon serum starvation of C2C12 myoblasts, FAK is transiently dephosphorylated in parallel with SHP-2 activation and association with FAK. SHP-2 knockdown by RNA interference suppressed the transient upregulation of SHP-2 and dephosphorylation of FAK during myogenesis. Furthermore, depletion of SHP-2 retarded the cell cycle withdrawal and the differentiation of serum-starved myoblasts into myotubes. These data provide a mechanistic basis for the reduction in FAK activity in differentiating myoblasts, indicating that myogenesis is critically triggered by FAK/SHP-2 complex. 583 18 2975 2981 Funda ao de Amparo a Pesquisa do Estado de Sao Paulo [2006/54878-3] Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Laboratorio Cristalia Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Funda ao de Amparo a Pesquisa do Estado de Sao Paulo [2006/54878-3] CNPq [305604/2006-6, 474650/2006-5]