Artículos de revistas
Microsatellites from rubber tree (Hevea brasiliensis) for genetic diversity analysis and cross-amplification in six Hevea wild species
Registro en:
Conservation Genetics Resources. Springer, v. 1, n. 1, n. 75, n. 79, 2009.
1877-7252
WOS:000207922400019
10.1007/s12686-009-9018-7
Autor
Souza, LM
Mantello, CC
Santos, MO
Goncalves, PD
Souza, AP
Institución
Resumen
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Hevea brasiliensis is native to the Amazonian rain forest and an important source of natural rubber. Twenty seven polymorphic microsatellite loci were isolated and characterized from a GA-CA enriched genomic library of H. brasiliensis. The number of alleles ranged from 2 to 20. The observed and expected heterozygosity ranged from 0.13 to 0.88 and from 0.00 to 0.89, respectively. Cross-species amplification of the markers developed for H. brasiliensis was successful in the wild Hevea species H. guianensis, H. rigidifolia, H. nitida, H. pauciflora, H. benthamiana and H. camargoana. The data indicated a high degree of sequence homology in the microsatellite flanking regions of these species. The developed SSR loci are a potential powerful tool for studies of population genetics, genetic diversity and gene flow among Hevea species. 1 1 75 79 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) FAPESP [2007/50392-1, 2007/59804-0]