ASPARAGINASE FROM THE TESTA OF DEVELOPING LUPIN AND PEA-SEEDS

dc.creatorSODEK, L
dc.creatorLEA, PJ
dc.date1993
dc.dateSEP
dc.date2014-12-16T11:36:07Z
dc.date2015-11-26T17:19:51Z
dc.date2014-12-16T11:36:07Z
dc.date2015-11-26T17:19:51Z
dc.date.accessioned2018-03-29T00:07:30Z
dc.date.available2018-03-29T00:07:30Z
dc.identifierPhytochemistry. Pergamon-elsevier Science Ltd, v. 34, n. 1, n. 51, n. 56, 1993.
dc.identifier0031-9422
dc.identifierWOS:A1993LX59100007
dc.identifier10.1016/S0031-9422(00)90781-7
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/54930
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/54930
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/54930
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1282997
dc.descriptionPotassium independent asparaginase has been purified from the testa of maturing seeds of Lupinus polyphyllus. The enzyme has a M(r) of 71 x 10(3) and is composed of two subunits of M(r) 35-36 x 10(3). Antisera raised against the purified asparaginase cross-reacted with a protein of similar characteristics isolated from the cotyledons and leaves of L. polyphyllus but no reaction was detected with extracts from the roots and nodules following Western blot analysis. Potassium dependent asparaginase was purified to a lesser extent from the testa of pea seeds and was shown to have a M(r) of 61 x 10(3). The potassium independent and dependent enzymes appear to be distinct proteins with different immunological characteristics. The proportion of the two enzymes varies with plant species, organ and developmental age.
dc.description34
dc.description1
dc.description51
dc.description56
dc.languageen
dc.publisherPergamon-elsevier Science Ltd
dc.publisherOxford
dc.publisherInglaterra
dc.relationPhytochemistry
dc.relationPhytochemistry
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectLUPINUS-POLYPHYLLUS
dc.subjectPISUM-SATIVUM
dc.subjectLEGUMINOSAE
dc.subjectASPARAGINASE
dc.subjectIMMUNOLOGY
dc.subjectPOTASSIUM
dc.subjectTESTA
dc.subjectPisum-sativum
dc.subjectAmino-acid
dc.subjectPolyacrylamide Gels
dc.subjectDeveloping Fruits
dc.subjectAmide Metabolism
dc.subjectDiurnal Changes
dc.subjectGarden Pea
dc.subjectGlutamine
dc.subjectCotyledons
dc.subjectLeaves
dc.titleASPARAGINASE FROM THE TESTA OF DEVELOPING LUPIN AND PEA-SEEDS
dc.typeArtículos de revistas


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