Artículos de revistas
Candida dubliniensis in a Brazilian family with an HIV 1-infected child: Identification, antifungal susceptibility, drug accumulation and sterol composition
Registro en:
Brazilian Journal Of Microbiology. Soc Brasileira Microbiologia, v. 37, n. 3, n. 237, n. 243, 2006.
1517-8382
WOS:000243335200008
Autor
de Melo, NR
Taguchi, H
Culhari, VVP
Sano, A
Fukushima, K
Miyaji, M
Manning, N
Kelly, SL
Vilela, MMS
Institución
Resumen
This study investigated the prevalence of C. dubliniensis in a Brazilian family with an HIV-infected child. A total of 42 oral isolates were obtained from eight family members. The identification of C. dubliniensis was performed by polymerase chain reactions (PCR) using primers against a specific sequence of the C. dubliniensis cytochrome b gene. Only the HIV-infected child and his grandmother were colonized by C. dubliniensis. In this study C. dubliniensis isolated from the HIV-infected child exhibited high susceptibility for azoles tested with MICs of 0.125 and 0.5 mu g/mL for voriconazole and fluconazole, respectively. Accumulation of [H-3] fluconazole in C. dubliniensis isolated from the HIV-infected child was slightly reduced in comparison to the reference susceptible strain. C. dubliniensis isolates had significantly lower ergosterol levels in comparison to C. albicans reference strains. 37 3 237 243