HPLC-ESI-MS/MS of imidazole alkaloids in Pilocarpus microphyllus

dc.creatorSawaya, ACHF
dc.creatorAbreu, IN
dc.creatorAndreazza, NL
dc.creatorEberlin, MN
dc.creatorMazzafera, P
dc.date2008
dc.dateJUL
dc.date2014-11-14T14:17:20Z
dc.date2015-11-26T17:15:11Z
dc.date2014-11-14T14:17:20Z
dc.date2015-11-26T17:15:11Z
dc.date.accessioned2018-03-29T00:03:26Z
dc.date.available2018-03-29T00:03:26Z
dc.identifierMolecules. Molecular Diversity Preservation Int, v. 13, n. 7, n. 1518, n. 1529, 2008.
dc.identifier1420-3049
dc.identifierWOS:000258142600007
dc.identifier10.3390/molecules13071518
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/68880
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/68880
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/68880
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1281983
dc.descriptionPilocarpine, an important imidazole alkaloid, is extracted from the leaves of Pilocarpus microphyllus ( Rutaceae), known in Brazil as jaborandi and used mainly for the treatment of glaucoma. Jaborandi leaves also contain other imidazole alkaloids, whose pharmacological and physiological properties are unknown, and whose biosynthetic pathways are under investigation. In the present study, a HPLC method coupled with ESI-MSn was developed for their qualitative and quantitative analysis. This method permits the chromatographic separation of the imidazole alkaloids found in extracts of jaborandi, as well as the MS/MS analysis of the individual compounds. Thus two samples: leaves of P. microphyllus and a paste that is left over after the industrial extraction of pilocarpine; were compared. The paste was found to contain significant amounts of pilocarpine and other imidazole alkaloids, but had a slightly different alkaloid profile than the leaf extract. The method is suitable for the routine analysis of samples containing these alkaloids, as well as for the separation and identification of known and novel alkaloids from this family, and may be applied to further studies of the biosynthetic pathway of pilocarpine in P. microphyllus.
dc.description13
dc.description7
dc.description1518
dc.description1529
dc.languageen
dc.publisherMolecular Diversity Preservation Int
dc.publisherBasel
dc.publisherSuíça
dc.relationMolecules
dc.relationMolecules
dc.rightsaberto
dc.sourceWeb of Science
dc.subjectjaborandi
dc.subjectPilocarpus microphyllus
dc.subjectimidazole alkaloids
dc.subjectpilocarpine
dc.subjectHPLC-ESI-MS/MS
dc.subjectLiquid-chromatographic Determination
dc.subjectIsopilocarpic Acid
dc.subjectDegradation Products
dc.subjectJaborandi
dc.subjectHydrochloride
dc.subjectColumn
dc.titleHPLC-ESI-MS/MS of imidazole alkaloids in Pilocarpus microphyllus
dc.typeArtículos de revistas


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