Artículos de revistas
Phenotypic evaluation of the effect of anaeroblosis on some virulence attributes of Candida albicans
Registro en:
Journal Of Medical Microbiology. Soc General Microbiology, v. 57, n. 10, n. 1277, n. 1281, 2008.
0022-2615
WOS:000259950400015
10.1099/jmm.0.2008/001107-0
Autor
Rosa, EAR
Rached, RN
Ignacio, SA
Rosa, RT
da Silva, WJ
Yau, JYY
Samaranayake, LP
Institución
Resumen
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) The current assumption that Candida albicans is a facultatively anaerobic organism has been widely accepted since its recovery from anoxic sites became common. However, the link between anaerobiosis and virulence remains uncertain. This study investigated the differential cell-surface hydrophobicity (CSH) using a hydrocarbon/water partition technique and analysed the differential secretion rates of secretory aspartyl proteases (Saps), esterase, chondroitinase and haemolysins of C. albicans strains recovered from periodontal pockets and non-periodontium-related intra-oral sites. For the enzymic tests, all strains from both sets were grown under aerobic and anaerobic conditions and the harvested cells were inoculated onto suitable normal or pre-reduced culture media in the presence or absence of molecular oxygen, respectively. The results showed that no variations were perceptible for CSH and chondroitinase (P >0.05). The secretion rates of esterase and haemolysins strongly decreased in an anoxic environment (P <0.0001). However, a consistent increment (P <0.0001) in Sap secretion was detected when Cultures were grown under anaerobic conditions. Based on these results, it is suggested that the oxygen concentration in the atmosphere surrounding cells exerts a variable influence on the virulence attributes of C. albicans. 57 10 1277 1281 Araucaria Foundation [9042] Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) OBU-PPDH-HKU Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Araucaria Foundation [9042] CAPES [BEX 0059/06-7]