Artículos de revistas
Extractive cultivation of xylanase by Penicillium janthinellum in a poly(ethylene glycol) cashew-nut tree gum aqueous two-phase system
Registro en:
Biotechnology Progress. Amer Chemical Soc, v. 20, n. 6, n. 1880, n. 1884, 2004.
8756-7938
WOS:000225558200037
10.1021/bp049746h
Autor
Oliveira, LA
Neto, BB
Porto, ALF
Tambourgi, EB
Institución
Resumen
Cultivation of the fungus Penicilliumjanthinellum for xylanase production was studied in a poly(ethylene glycol)/cashew-nut tree gum aqueous two-phase system, using a two-level fractional factorial design. The parameters studied were initial pH, cultivation time, type of agro-industrial residue (oat husk or corn cob), agitation, temperature, and phase-forming polymers. The xylanase produced during fermentation partitioned into the top phase. The agitation and temperature (negative), cultivation time and initial pH (positive) effects proved statistically significant for xylanase production. The highest percentage yield of the xylanase in the top and its production in the top phase, about 97% and 160.7 U/mL, were obtained in cultures of 120 h, 40 rpm, 25 degreesC, and pH 5.0. 20 6 1880 1884