Artículos de revistas
Dact Gene Expression Profiles Suggest a Role for This Gene Family in Integrating Wnt and TGF-beta Signaling Pathways During Chicken Limb Development
Registro en:
Developmental Dynamics. Wiley-blackwell, v. 243, n. 3, n. 428, n. 439, 2014.
1058-8388
1097-0177
WOS:000331374000007
10.1002/dvdy.23948
Autor
Sensiate, LA
Sobreira, DR
Da Veiga, FC
Peterlini, DJ
Pedrosa, AV
Rirsch, T
Joazeiro, PP
Schubert, FR
Collares-Buzato, CB
Xavier-Neto, J
Dietrich, S
Alvares, LE
Institución
Resumen
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Background:Dact gene family encodes multifunctional proteins that are important modulators of Wnt and TGF- signaling pathways. Given that these pathways coordinate multiple steps of limb development, we investigated the expression pattern of the two chicken Dact genes (Dact1 and Dact2) from early limb bud up to stages when several tissues are differentiating.Results: During early limb development (HH24-HH30) Dact1 and Dact2 were mainly expressed in the cartilaginous rudiments of the appendicular skeleton and perichondrium, presenting expression profiles related, but distinct. At later stages of development (HH31-HH35), the main sites of Dact1 and Dact2 expression were the developing synovial joints. In this context, Dact1 expression was shown to co-localize with regions enriched in the nuclear -catenin protein, such as developing joint capsule and interzone. In contrast, Dact2 expression was restricted to the interzone surrounding the domains of bmpR-1b expression, a TGF- receptor with crucial roles during digit morphogenesis. Additional sites of Dact expression were the developing tendons and digit blastemas.Conclusions: Our data indicate that Dact genes are good candidates to modulate and, possibly, integrate Wnt and TGF- signaling during limb development, bringing new and interesting perspectives about the roles of Dact molecules in limb birth defects and human diseases. Developmental Dynamics 243:428-439, 2014. (c) 2013 Wiley Periodicals, Inc. 243 3 428 439 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) AFM (The Association Francaise Contre les Myopathies Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) FAPESP [2006/05892-3]