Relaxation of the mouse pubic symphysis during late pregnancy is not accompanied by the influx of granulocytes

dc.creatorRosa, RG
dc.creatorTarsitano, CAB
dc.creatorHyslop, S
dc.creatorYamada, AT
dc.creatorToledo, OMS
dc.creatorJoazeiro, PP
dc.date2008
dc.dateMAR
dc.date2014-11-19T11:12:12Z
dc.date2015-11-26T17:05:25Z
dc.date2014-11-19T11:12:12Z
dc.date2015-11-26T17:05:25Z
dc.date.accessioned2018-03-28T23:53:48Z
dc.date.available2018-03-28T23:53:48Z
dc.identifierMicroscopy Research And Technique. Wiley-liss, v. 71, n. 3, n. 169, n. 178, 2008.
dc.identifier1059-910X
dc.identifierWOS:000254056000001
dc.identifier10.1002/jemt.20549
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/70838
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/70838
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/70838
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1279583
dc.descriptionIn some animals, such as mice and guinea pigs, a hormonally controlled mechanism increases the flexibility of the pubic symphysis and enhances the cervical remodeling necessary for safe delivery. Cervical ripening during pregnancy is associated with a paradoxical influx of leukocytes. However, the changes in cell metabolism during relaxation of the mouse pubic symphysis for delivery have not been extensively studied. In this work, we used light microscopy and transmission and scanning electron microcopy, as well as immunohistochemistry and Western blotting for MMP-8, to investigate the involvement of granulocytes or resident stromal cells in the relaxation of the virgin pubic symphysis during late pregnancy (days 18 and 19, before delivery) in vivo and in explanted joints. MMP-8 was studied because this collagenase is a hallmark for cervical ripening associated with the influx of granulocytes during late pregnancy. Extensive dissolution and disorganization of the extracellular matrix was seen around fibroblastic-like cells in late pregnancy. In contrast to the cervix (positive control), morphological and immunohistochemical analyses revealed that there was no characteristic cellular inflammatory response in the interpubic tissue. Staining for MMP-8 was observed in chondroid and fibroblastic-like cells of virgin and relaxed interpubic ligament, respectively. However, no granulocytes were seen during the extensive remodeling of the pubic joint in late pregnancy. These results indicate that constitutive stromal cells may have an important role in tissue relaxation during remodeling of the pubic symphysis in pregnancy.
dc.description71
dc.description3
dc.description169
dc.description178
dc.languageen
dc.publisherWiley-liss
dc.publisherHoboken
dc.publisherEUA
dc.relationMicroscopy Research And Technique
dc.relationMicrosc. Res. Tech.
dc.rightsfechado
dc.rightshttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dc.sourceWeb of Science
dc.subjectextracellular matrix
dc.subjectmouse pubic symphysis
dc.subjectmetalloproteinase
dc.subjectremodeling
dc.subjectMatrix Metalloproteinase-8
dc.subjectPolymorphonuclear Leukocytes
dc.subjectInflammatory Mediators
dc.subjectCollagen-metabolism
dc.subjectRipening Process
dc.subjectCervical Tissue
dc.subjectUterine Cervix
dc.subjectRelaxin
dc.subjectParturition
dc.subjectExpression
dc.titleRelaxation of the mouse pubic symphysis during late pregnancy is not accompanied by the influx of granulocytes
dc.typeArtículos de revistas


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