Solubilization of human erythrocyte membranes by non-ionic surfactants of the polyoxyethylene alkyl ethers series

dc.creatorPrete, PSC
dc.creatorGomes, K
dc.creatorMalheiros, SVP
dc.creatorMeirelles, NC
dc.creatorde Paula, E
dc.date2002
dc.dateMAY 23
dc.date2014-11-19T10:58:43Z
dc.date2015-11-26T17:05:21Z
dc.date2014-11-19T10:58:43Z
dc.date2015-11-26T17:05:21Z
dc.date.accessioned2018-03-28T23:53:43Z
dc.date.available2018-03-28T23:53:43Z
dc.identifierBiophysical Chemistry. Elsevier Science Bv, v. 97, n. 1, n. 45, n. 54, 2002.
dc.identifier0301-4622
dc.identifierWOS:000176292600005
dc.identifier10.1016/S0301-4622(02)00042-X
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/71834
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/71834
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/71834
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1279563
dc.descriptionIn the present study, we investigated the interaction of the non-ionic surfactants polyoxyethylene alkyl ethers (CnEm) with erythrocyte membranes. For this purpose we have performed hemolytic assays under isosmotic conditions with five surfactants in the 8 polyoxyethylene ether series. By applying to the hemolytic curves a quantitative treatment developed for the study of surface-active compounds on biomembranes, we could calculate the surfactant/lipid molar ratios for the onset of hemolysis (R-e(sat)) and for complete hemolysis (R-e(sol)). This approach also allowed the calculation of the binding constants for each surfactant to the erythrocyte membrane. Results in the C,,E series were compared to those obtained for Triton X-100, a well-known non-ionic surfactant with values of cmc and HLB in the range of the alkyl ethers studied. Inside the series the lytic effect increased with the more hydrophobic homologues (C10E8 < C12E8 < C14E8 < C16E8 < C18E8), with Re values between 3:1 and 0.03:1. The effect of C10E8 and C12E8 was found to be in the range of that caused by Triton X-100, proving that C,,E surfactants are strongly hemolytic. (C) 2002 Elsevier Science B.V. All rights reserved.
dc.description97
dc.description1
dc.description45
dc.description54
dc.languageen
dc.publisherElsevier Science Bv
dc.publisherAmsterdam
dc.publisherHolanda
dc.relationBiophysical Chemistry
dc.relationBiophys. Chem.
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjecterythrocyte
dc.subjectmembrane
dc.subjectsurfactants
dc.subjecthemolysis
dc.subjectsolubilization
dc.subjectNonionic Surfactants
dc.subjectBiological-membranes
dc.subjectHemolytic-activity
dc.subjectShape Changes
dc.subjectTriton X-100
dc.subjectDifferential Solubilization
dc.subjectLytic Concentrations
dc.subjectMixed Micelles
dc.subjectChain-length
dc.subjectDetergents
dc.titleSolubilization of human erythrocyte membranes by non-ionic surfactants of the polyoxyethylene alkyl ethers series
dc.typeArtículos de revistas


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