Artículos de revistas
Utilization of the etest assay for comparative antibiotic susceptibility profiles of citrus variegated chlorosis and Pierce's disease strains of Xylella fastidiosa
Registro en:
Current Microbiology. Springer, v. 51, n. 4, n. 262, n. 266, 2005.
0343-8651
WOS:000232401200010
10.1007/s00284-005-0015-0
Autor
Ribeiro, MD
Dellias, MD
Tsai, S
Bolmstrom, A
Meinhardt, LW
Bellato, CD
Institución
Resumen
Xylella fastidiosa has a wide host range. Isolates of this bacterium that cause diseases in citrus (CVC) and grapes (PD) share 98% genome homology, and 95.7% amino acid identity. Drug resistance genes show a higher level of divergence and may be involved in the X. fastidiosa-host interaction. Antibiotic susceptibility of CVC and PD strains were compared utilizing the Etest strip method (AB Biodisk). Etest is applicable for fastidious slow-growing organisms due to its reproducibility. Results showed that the CVC strain was resistant to bacitracin, cefotaxime, and trimethoprim, and susceptible to chloramphenicol, erythromycin, gentamicin, kanamycin, streptomycin, and tetracycline. The PD strain was susceptible to all tested antibiotics, except kanamycin and trimethoprim. Both isolates produced a class C beta-lactamase. These data support previous antibiotic studies and gene discrepancies found in the sequencing data of PD and CVC strains. These results demonstrate the efficacy of utilizing Etest assays for X. fastidiosa strains. 51 4 262 266