Artículos de revistas
A new laboratorial method for the diagnosis of gastrointestinal parasites in dogs
Registro en:
Revista Brasileira De Parasitologia Veterinaria. Brazilian Coll Veterinary Parasitology, v. 22, n. 1, n. 1, n. 5, 2013.
1984-2961
WOS:000322556500002
Autor
Coelho, WMD
Gomes, JF
do Amarante, AFT
Bresciani, KDS
Lumina, G
Koshino-Shimizu, S
Leme, DP
Falcao, AX
Institución
Resumen
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) In this study, we aimed to introduce a new technique called TF-Test Modified/Dog for the diagnosis of gastrointestinal parasites in dogs. Fecal samples from 106 dogs were processed by the technique TF-Test Modified/Dogand the techniques of centrifugation-flotation in zinc sulfate, simple-flotation by saturated solution of sodium chloride, direct microscopy exam and TF-Test Conventional. Sensitivity was higher in the TF-Test Modified/Dog (98.41%), followed by flotation in saturated zinc sulfate (77.78%), TF-Test Conventional (73.02%), flotation by saturated sodium chloride (55.55%), and direct microscopy exam (30.16%). The diagnostic efficiency varied from 58.49% to 99.06%, with the highest value also obtained by the new proposed technique. Efficiency level of 99.06% with kappa index 0.979 (almost perfect) was obtained with the TF-Test Modified/Dog. These results represent significant statistical gains (P < 0.05) of 20.63% in sensitivity and 12.27% in efficiency over the best among the other techniques flotation by saturated zinc sulfate, whose kappa index was 0.738, much lower than that of the TF-Test Modified/Dog. All techniques presented 100% specificity. In this sense, the high sensitivity of the TF-Test Modified/Dog makes it suitable for epidemiological surveys of gastrointestinal parasitosis in dogs, zoonoses control and preventive surveillance programs. 22 1 1 5 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) FAPESP [2008/57428-4, 2008/50090-8]