The homozygous inheritance of recessive alleles for lipoxygenase, -L1, -L2 and -L3 incorporated into a commercial soya bean seed line, IAC 8, was evaluated. The activity of each isozyme, determined by spectrophotometry demonstrated a very low activity for the mutant -L1 and respective progeny and a low activity for -L2, -L3 and their progenies. The methodology employed was shown to be a reliable and rapid method when evaluating lipoxygenase from crops.622207208