Artículos de revistas
Purification of a Kunitz-type Inhibitor from Acacia polyphylla DC Seeds: Characterization and Insecticidal Properties against Anagasta kuehniella Zeller (Lepidoptera: Pyralidae)
Registro en:
Journal Of Agricultural And Food Chemistry. Amer Chemical Soc, v. 61, n. 10, n. 2469, n. 2478, 2013.
0021-8561
WOS:000316243900022
10.1021/jf3049565
Autor
Machado, SW
de Oliveira, CFR
Bezerra, CDS
Freire, MDM
Kill, MR
Machado, OLT
Marangoni, S
Macedo, MLR
Institución
Resumen
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Anagasta kuehniella is a polyphagous pest that causes economic losses worldwide. This species produces serine proteases as its major enzymes for protein digestion. In this study, a new serine-protease inhibitor was isolated from Acacia polyphylla seeds (AcKI). Further analysis revealed that AcKI is formed by two polypeptide chains with a relative molecular mass of similar to 20 kDa. The effects of AcKI on the development, survival, and enzymatic activity of Anagasta kuehniella larvae were evaluated, by incorporating AcKI in an artificial diet Bioassays revealed a reduction in larval weight of similar to 50% with the lower concentration of AcKI used in the study (0.5%). Although additional assays showed an increase in endogenous trypsin and chymotrypsin activities, with a degree of AcKI-insensivity, AcKI produces an anti nutritional effect on A. kuehniella, indicating AcKI as a promising bioinsecticide protein for engineering plants that are resistant to insect pests. 61 10 2469 2478 FINEP Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) FUNDECT Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)