The Langmuir model fitted well the adsorption isotherms of lipase on the hydrophobic resin. The model parameters, Q(m) and k(d), were affected by NaCl concentration: Q(m) increased from 31 to 80 U g(-1) resin, and k(d) changed from 9.4 to 3 U ml(-1). Column modelling and the simulation data were compared with the experimental data with good agreement. The highest achieved column efficiency was 71%.2310781786