Cytotoxicity of Lachesis muta muta snake (bushmaster) venom and its purified basic phospholipase A(2) (LmTX-I) in cultured cells

dc.creatorDamico, DCS
dc.creatorNascimento, JM
dc.creatorLomonte, B
dc.creatorPonce-Soto, LA
dc.creatorJoazeiro, PP
dc.creatorNovello, JC
dc.creatorMarangoni, S
dc.creatorCollares-Buzato, CB
dc.date2007
dc.dateAPR
dc.date2014-11-18T17:32:01Z
dc.date2015-11-26T16:57:32Z
dc.date2014-11-18T17:32:01Z
dc.date2015-11-26T16:57:32Z
dc.date.accessioned2018-03-28T23:45:06Z
dc.date.available2018-03-28T23:45:06Z
dc.identifierToxicon. Pergamon-elsevier Science Ltd, v. 49, n. 5, n. 678, n. 692, 2007.
dc.identifier0041-0101
dc.identifierWOS:000245866100010
dc.identifier10.1016/j.toxicon.2006.11.014
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/57642
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/57642
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/57642
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1277691
dc.descriptionHuman envenoming by Lachesis muta muta venom, although infrequent, is rather severe, being characterized by pronounced local tissue damage and systemic dysfunctions. Studies on the pharmacological actions of L. m. muta venom are relatively scant and the direct actions of the crude venom and its purified phospholipase A(2) (PLA(2) ) have not been addressed using in vitro models. In this work, we investigated the cytotoxicity of L. m. muta venom and its purified PLA(2) isoform LmTX-1 in cultured Madin-Darby canine kidney (MDCK) and in a skeletal muscle (C2C12) cell lines. As revealed by neutral red dye uptake assay, the crude venom (10 or 100 mu g/ml) induced a significant decrease in cell viability of MDCK cells. LmTX-I at the concentrations tested (70-270 mu g/ml or 5-20 mu M) displayed no cytotoxicity in both MDCK and C2C12 cell lines. Morphometric analysis of Feulgen nuclear reaction revealed a significant increase in chromatin condensation (pyknosis), apparent reduction in the number of mitotic nuclei and nuclear fragmentation of some MDCK cells after incubation with L. m. muta venom. Monolayer exposure to crude venom resulted in morphological changes as assessed by scanning electron microscopy. The staining with TRITC-labelled phalloidin showed a marked disarray of the actin stress fiber following L. in. muta venom exposure. In contrast, LmTX-1 had no effect on nucleus and cell morphologies as well as on stress fiber organization. These results indicate that L. m. muta venom exerts toxic effects on cultured MDCK cells. The LmTX-I probably does not contribute pet, se to the direct venom cytotoxicity, these effects are mediated by metalloproteinases/disintegrins and other components of the venom. (c) 2006 Elsevier Ltd. All rights reserved.
dc.description49
dc.description5
dc.description678
dc.description692
dc.languageen
dc.publisherPergamon-elsevier Science Ltd
dc.publisherOxford
dc.publisherInglaterra
dc.relationToxicon
dc.relationToxicon
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectLachesis muta muta
dc.subjectsnake venom
dc.subjectcytotoxicity
dc.subjectMDCK cells
dc.subjectphospholipase A(2)
dc.subjectAmino-acid Oxidase
dc.subjectApoptosis-inducing Factor
dc.subjectCobra Ophiophagus Hannah
dc.subjectLocal Tissue-damage
dc.subjectSkeletal-muscle
dc.subjectLhf-i
dc.subjectStructural Determinants
dc.subjectBiological-activities
dc.subjectProtein Expression
dc.subjectHemorrhagic Factor
dc.titleCytotoxicity of Lachesis muta muta snake (bushmaster) venom and its purified basic phospholipase A(2) (LmTX-I) in cultured cells
dc.typeArtículos de revistas


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